产葡聚糖明串珠菌的分离鉴定及发酵条件研究

Isolation and Identification of Dextran-Producting Strain and Study of Fermentation Conditions

DOI：10.3969/j.issn.1673-1689.2012.07.009

中文关键词: 分离 鉴定 葡聚糖 肠膜明串珠菌 正交试验

英文关键词: Isolation identification dextran Leuconostoc mesenteroides orthogonal experiment

基金项目:国家863计划项目(2011AA100903)

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中文摘要:

从实验室保存的开菲尔粒中分离筛选高产葡聚糖菌株。经乳酸菌的初筛和胞外多糖产糖量分析,筛选出一株能高产葡聚糖的菌株,命名T-L1,结合形态学特征和16SrRNA的序列分析,将该菌株鉴定为明串珠菌(Leuconostoc mesenteroides),采用单因素和正交试验对该菌发酵条件进行优化研究,结果表明该菌的最佳发酵条件发酵温度为28℃、发酵时间为24h、接种量体积分数为5%,培养基起始pH值为6.5。在此优化条件下,葡聚糖的产量为30.12g/L,比优化前提高了41%。

英文摘要:

In this study,a dextran-producting strain T-L1,was isolated from kefir by by lactobacillus screening and dextran yeild analysis.Then strain T-L1 was identified as Leuconostoc mesenteroides by the morphological properties and 16S rRNA sequence.Furthermore,the single factor experiments and orthogonal experiments were adopted to optimize the nutrients and environmental conditions for increasing production of dextran by strain T-L1.The optimum conditions for dextran production listed as follows: the initial pH 6.5,the fermentation temperature 28 ℃,the inoculation volume 5% and the fermentation time 24 h.Under the optimum condtions,the titer of dextran was achieved at 30.12 g/L,higher 41.08% than that of the control.

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