一株微好氧趋磁细菌TH-1的分离及其磁小体研究

Isolation of a Microaerobic Magnetotactic Bacterium TH-1 and Studies on the Magnetosome

DOI：10.3969/j.issn.1673-1689.2012.06.012

中文关键词: 趋磁细菌 纳米材料 磁小体

英文关键词: magnetotactic bacteria nano-material magnetosome

基金项目:国家自然科学基金项目(20806033);张家港市科技局基金项目(ZKS0904)

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中文摘要:

研究生物磁学的性质,从无锡太湖水域中分离到了一株沿着磁力线运动的微好氧细菌-趋磁细菌TH-1。尽管在其他淡水中曾经分离过到过趋磁细菌,但却从未在太湖中分离到过,因此TH-1是目前所见报道的第一株分离自太湖的趋磁细菌。采用透射电镜的方法对细菌进行了形态学观察,结果表明,每个细胞内含多个磁小体,呈圆形,直径范围在10～100 nm,分布在细胞质以及细胞壁前端。能谱结果显示,该菌磁小体的元素组成为铁(Fe)、硫(S)和氧(O)。还对该菌以及磁小体的磁性进行了分析并且对菌株做了初步的生化鉴定。结果表明,分离得到的菌株隶属于趋磁细菌。

英文摘要:

In order to study the properties of biomagnetism,a kind of magnetotactic bacteria TH-1 which is able to respond and orient along the lines of terrestrial or artificial magnmetic fields was isolated from Taihu lake.TH-1 is the first reported magnetotactic bacterium isolated from Taihu lake.Morphological observation was carried out using the transmission electron microscope and the results showed that magnetic cells each possess several magnetosomes that is circular-shaped and 10 nm-100 nm in diameter,which distributed in cytoplasm and the front cell wall.The Energy dispersive X-ray spectrum of magnetosome indicates that stain TH-1 consists of three mineral elements Fe,S and O.The magnetic properties both of the bacteria and magnetosomes were studied and a preliminary biochemical identification of the bacteria was carried out.All the above results demonstrated that strain TH-1 is magnetotactic bacteria.

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