土曲霉与红曲霉双灭活原生质体融合选育洛伐他汀高产菌株

Studies on the Breeding of High Lovastatin-Producing Strains by Fusion of Inactivated Protoplasts from Aspergillus terreus and Monascus anka

DOI：10.3969/j.issn.1673-1689.2012.02.011

中文关键词: 洛伐他汀 红曲霉 土曲霉 原生质体融合

英文关键词: lovastatin Monascus anka Aspergillus terreus protoplast fusion

基金项目:广州市科技计划项目(2010Y1-C811)

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中文摘要:

研究原生质体融合技术选育洛伐他汀高产菌株的方法。土曲霉(Aspergillus terreus)和红曲霉(Monascus anka)用混合酶液(体积分数0.5%溶菌酶,0.3%纤维素酶,0.3%蜗牛酶)分别酶解5.0h和3.5h制备原生质体,土曲霉原生质体和红曲霉原生质体在紫外线(20 W,30cm)下分别照射5min和4min灭活,灭活后混合并用PEG(体积分数30%PEG,0.05mol/LCaCl2,0.05mol/L甘氨酸)介导融合。在187株融合子中筛选得到27株洛伐他汀产量高于红曲霉出发菌株的融合子,其中有7株的洛伐他汀产量是出发菌的2倍以上。双灭活原生质体融合方法成功选育出红曲霉洛伐他汀高产菌株。

英文摘要:

In order to isolate a high lovastatin-producing Monascus strains,protoplast fusion technology was introduced in this manuscript.The protoplasts of Aspergillus terreus and Monascus anka was prepared under 5 hour and 3.5 hour enzymolysis,respectively,by mixed enzymes(0.5% lysozyme +0.3% Cellulase +0.3% snail enzyme) and then inactive by ultraviolet irradiation(20 W,30 cm)for 5 min and 4 min,respectively and mixed with PEG(30% PEG,0.05mol/L CaCl2,0.05 mol/L glycine).27 high lovastatin-producing strains were obtained from 187 fusants.Seven of them exhibited more than twice the yield of the original Monascus strain.

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