羧基化磁性纳米微球的表面生物修饰方法

Surface Biological Modification Research on Carboxylated Magnetic Nanoparticles

DOI：10.3969/j.issn.1673-1689.2012.01.011

中文关键词: 磁珠 生物修饰 EDC 免疫层析

英文关键词: magnetic nanoparticles biological modification EDC lateral flow immunoassay

基金项目:“十一五”国家科技支撑计划项目(2008BAD94B09);上海市教育委员会优秀青年教师基金项目(B-8101-09-0036);上海市教育委员会重点学科建设项目(J50704);上海市科技兴农重点攻关项目(沪农科攻字(2009)第6-1号)

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中文摘要:

采用A和B两种羧基化磁珠,以山羊抗小鼠IgG为模式蛋白,优化了EDC/sulfo-NHS法的羧基磁珠表面抗体的修饰条件,对比分析了抗体修饰前后磁珠粒径和多分散系数(PDI)的变化,并利用竞争性免疫层析法评价了磁珠表面修饰抗体的生物学活性。优化结果显示,不同粒径和厂家的羧基磁珠,其表面的最佳抗体修饰条件是不同的。磁珠A抗体修饰前后的平均水力学粒径变化率为6.55%,PDI分别为0.011和0.046,变化微小,而磁珠B在抗体修饰前后的平均水力学粒径变化率为135.55%,分散性能变差。生物学活性评价结果显示优化条件下制备的磁珠A和B表面的抗体均具有较好的抗原结合活性,磁珠B的磁信号强于磁珠A。综上所述,抗体修饰磁珠用于免疫层析检测时,抗体修饰前后磁珠粒径和分散性能的变化、材料的磁响应性能对于检测灵敏度和层析速度具有重要意义。

英文摘要:

Using goat anti-mouse IgG monoclonal antibody as model protein,antibody modification conditions on the surface of carboxylated superparamagnetic nanoparticles(SMN) A and B were optimized,then hydrodynamic sizes and polydispersity index(PDI) of SMN-A and B before and after antibody modification were compared.Additionally,the biological activity of modifying antibodies was assessed by a competitive format lateral flow immunoassay(LFIA).Optimization results indicated that the best modification conditions were different for SMNs of different sizes and manufactures.Before and after modification,the hydrodynamic size change rate of SMN-A was 6.55%,PDI were 0.011 and 0.046,respectively,the change was minor,while hydrodynamic size change rate of SMN-B was 135.5%,and the dispersivity became worse.Furthermore,the antibody modifying on SMN-A and B both showed good biological activities by LFIA test,and SMN-A indicated better lateral flow performance.Briefly,if antibody modifying SMNs were chosen as labels in LFIA test,size and dispersivity change before and after modification,magnetic signal intensity of the material had great mean in improving detection sensitivity and assay rate.

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