几种酚酸对木聚糖酶活力的影响

Influence of Phenolic Acids on Activity of Xylanase

DOI：10.3969/j.issn.1673-1689.2012.01.015

中文关键词: 酚酸 木聚糖酶 酶活力

英文关键词: phenolic acid xylanase activity

基金项目:广东高校科技成果产业化重大项目(cgzhzd0709)

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中文摘要:

采用3,5-二硝基水杨酸法(DNS法)测定酶解后还原糖释放量,研究4种不同酚酸(阿魏酸、对-香豆酸、水杨酸、单宁酸)对木聚糖酶活力的影响。结果表明:阿魏酸、对-香豆酸、水杨酸能提高木聚糖酶活力,当这3种酚酸质量浓度为0.75mg/mL时,酶活力分别提高65.59%、46.21%和12.83%。单宁酸抑制木聚糖酶活性,添加量为0.50mg/mL时,抑制率达37.18%。动力学研究表明:上述4种酚酸均能提高酶与底物的亲和力,对-香豆酸能提高酶反应速度,但单宁酸会使反应速度显著降低。

英文摘要:

In this manuscript,effect of four phenolic acids,including ferulic,coumaric,salicylic and tannic acid,on the activity of xylanase was investigated through determination of reducing sugars releasing from xylans.The results showed that 0.75 mg/mL ferulic,coumaric and salicylic acid could increased the activity of xylanase by 65.59%,46.21% and 12.83%,respectively.However,tannic acid was found as inhibitor of xylanase,and the activity decreased 37.18% when 0.50 mg/mL was presented.The results of enzyme kinetics showed that these phenolic acids increased the affinity of xylanase for the substrate.The reaction speed increased with coumaric acid added,and significantly reduced by tannic aci

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