大豆异黄酮和皂甙对扑热息痛诱导的小鼠急性肝损伤的保护作用

Protective Effects of Soy Isoflavones and Saponins on Acute Liver Injury Induced by Acetaminophen in Mice

DOI：10.3969/j.issn.1673-1689.2013.12.005

中文关键词: 大豆 异黄酮 皂甙 肝损伤 抗炎 抗氧化

英文关键词: soy isoflavones saponins liver injury anti-inflammatory anti-oxidative

基金项目:国家自然科学基金项目(30360113;81160539)

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中文摘要:

研究了大豆异黄酮和皂甙对扑热息痛(APAP)诱导的小鼠急性肝损伤的保护作用。将实验小鼠随机分为正常组、模型组、大豆异黄酮组、大豆皂甙组及联苯双酯组(阳性对照组)。每日给药1次,连续7 d。实验末期,腹腔注射APAP建立急性肝损伤模型,比色法检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、白蛋白(ALB)以及肝脏诱导型一氧化氮合酶(iNOS)、一氧化氮(NO)、过氧化脂质(LOOH)、丙二醛(MDA)、还原型谷胱甘肽(GSH)含量和过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)的活性。结果表明,大豆异黄酮和皂甙显著降低APAP诱发的急性肝损伤小鼠血清ALT、AST和ALP活性,增高血清ALB水平,降低肝iNOS活性和NO水平,减少肝LOOH和MDA含量,升高肝组织CAT、GPx、SOD活性和GSH水平。提示,大豆异黄酮和皂甙对APAP诱导的小鼠急性肝损伤具有保护作用,其机制可能与抗炎和抗氧化作用有关。

英文摘要:

The protective effect of soy isoflavones and saponins on acute liver injury induced by acetaminophen(APAP) in mice was investigated in this manuscript. The mice were randomly assigned to the normal control,model control,soy isoflavone,saponin and bifendate(positive control) groups. Animals were treated once daily for 7 days. APAP were given intraperitoneally to the mice of groups,then the alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP),albumin(ALB),inducible nitric oxide synthase(iNOS),nitric oxide(NO),lipid hydroperoxide(LOOH),malondialdehyde(MDA),glutathione(GSH),catalase(CAT),glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD) were detected by the colorimetric method. The administration with soy isoflavones and saponins could(1) reduced the serum ALT,AST and ALP;(2) increased the serum ALB level;(3)decreased hepatic iNOS activity and NO level,(4) decreased the hepatic LOOH and MDA contents,and(5)increased the CAT,GPx,SOD activities and GSH level of liver in mice with acute liver injury. Those results demonstrated that soy isoflavones and saponins have protective effects on acute liver injury induced by APAP in mice, probably via anti-inflammatory and nti-oxidative activities.

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