圆酵母B84512单倍体的制备及其产赤藓糖醇特性分析

Breeding of Torula sp. B84512 Haploid and Analysis of Erythritol Production Characteristics

DOI：10.3969/j.issn.1673-1689.2013.12.015

中文关键词: 圆酵母B84512 单倍体 子囊孢子 赤藓糖醇

英文关键词: Torula sp.B84512 haploid ascospore erythritol

基金项目:江苏省科委社会发展基金项目(BE2010629)

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中文摘要:

圆酵母B84512为工业用赤藓糖醇生产菌株,为获得其遗传育种单倍体亲本,以Mcclary产孢培养基进行该菌株子囊孢子萌发,萌发条件为:30℃,培养7 d,菌株产孢率为45%;以蜗牛酶裂解子囊孢子细胞壁150 min,共筛选出10株单倍体菌株,其中3株在发酵培养基中合成赤藓糖醇的能力相对较高,分别命名为Torula sp.B84512-7,Torula sp.B84512-8及Torula sp.B84512-9。经PCR验证,前两者为α型,后者为a型。将3株单倍体两两杂合,发现杂合子Torula sp.B84512-79的发酵性能最佳,赤藓糖醇产量高达97 g/L,约为出发菌株的56%。赤藓糖醇产量较高的单倍体亲本Torula sp.B84512-7及Torula sp.B84512-9可用于基因工程育种。

英文摘要:

The Torula sp. B84512 was used for the industrial production of erythritol. To obtain a haploid genetics hybrid breeding of Torula sp. B84512,spore-producing cultivation of B84512 were done after cultured on Mcclary medium for 7 days at 30 ℃,and the sporulation rate could reach to 45%. Lysing the ascospore cell with snail enzyme for 150 min,10 haploid strains were screened. Furthermore,3 of them exhibit the highest ability to produce erythritol,and named as Torula sp. B84512-7,B84512-8 and B84512-9,respectively. With the PCR verification,Torula sp. B84512-7 and B84512-8 were verified as alpha type but Torula sp. B84512-9 was a type. The erythritol production of heterozygous Torula sp. B84512-79 reached at 97 g/L which was 56% of that of the parental strain. Therefore,Torula sp. B84512-7 and Torula sp. B84512-9 could be used for the further genetic manipulation.

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