血红密孔菌(Pycnoporus sanguineus SYBC-L7)色素的提取及其理化性质
Extraction and Physicochemical Property of Pigment from Pycnoporus sanguineus SYBC-L7
DOI:10.3969/j.issn.1673-1689.2013.11.007
中文关键词: 色素 固态发酵 提取 稳定性 血红密孔菌
英文关键词: pigment solid state fermentation extraction stability Pycnoporus sanguineus
基金项目:国家自然科学基金项目(21176106);国家863计划项目(2010AA101501);国家重大科技支撑与自主创新专项引导资金项目(BY2010117)
作者
单位
骆守鹏
江南大学工业生物技术教育部重点实验室,江苏无锡,214122
范晶晶
毛飞君
金坤生物工程有限公司,江苏无锡,214131
蔡宇杰
廖祥儒
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中文摘要:
研究了一株血红密孔菌(Pycnoporus sanguineus SYBC-L7)固态发酵产色素的提取条件及色素的理化性质。结果表明:该菌株在以木屑为基质固态发酵时色素产量最高;以甲醇为提取液,在35℃水浴下40 min后可从发酵后的培养物中最大化提取该色素;所得色素溶液呈黄色,在可见光区的最大吸收波长为430 nm,该色素的热稳定性和耐酸性较好,常用的氧化剂与还原剂对其影响不大。
英文摘要:
The optimum conditions of extracting pigment from the solid state fermentation culture of Pycnoporus sanguineus SYBC-L7 were studied,and the physicochemical properties of this pigment were also analyzed. The results showed that the pigment could be optimum produced by solid-state fermentation with wood chips. Using methanol as the extraction solvent,the pigment could be maximally extracted from the solid culture at 35 ℃ after 40 min with water bath. The extraction solvent was yellow with a maximum absorption wavelength of 430 nm. The pigment has a good thermal resistance as well as acid resistance,and it cannot be affected by common oxidizing and reducing agents.
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