响应曲面法优化黄参茎叶多酚的提取工艺及其抗氧化活性

Response Surface Methodology for Optimization Extraction Process of Sphallerocarpus gracilis Stem Leaves Polyphenol and Determination of Antioxidant Activity

DOI：10.3969/j.issn.1673-1689.2013.11.016

中文关键词: 黄参茎叶 多酚 响应曲面法 超声波辅助提取 抗氧化活性

英文关键词: Sphallerocarpus gracilis stem leaves polyphenol response surface method ultrasonic assisted extraction antioxidation

基金项目:陕西师范大学研究生培养创新基金项目(2013CXS005)

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中文摘要:

研究黄参茎叶多酚的超声波辅助提取工艺及抗氧化活性,以响应曲面法优化黄参茎叶多酚的超声波辅助提取工艺,同时以分光光度法测定提取物的还原力,对羟基自由基(·OH)、DPPH自由基(DPPH·)的清除作用。结果表明:黄参茎叶多酚的最佳提取工艺条件为:乙醇体积分数75%、提取温度70℃、料液比(g/mL)1∶35、提取时间30 min,此时多酚得率为1.1448%;黄参茎叶多酚对羟基自由基、DPPH自由基具有良好的清除能力,同时还具有一定的还原能力,并且在0.1~0.6 mg/mL质量浓度范围内,黄参茎叶多酚对羟基自由基、DPPH自由基的清除率高于BHT,表明其具有良好的抗氧化作用,可作为天然抗氧化剂进一步开发和利用。

英文摘要:

In order to study the Ultrasound-assisted extraction technology of Sphallerocarpus gracilis stem leaves polyphenol and antioxidant activity. Response surface methodology(RSM) was used to establish the mathematical model of solvent extraction extraction to obtain the optimum conditions. The antioxidant activity of the extraction was evaluated by spectrophotometry on its ability of scavenging hydroxyl radical(·OH),1-diphenyl-2-picrylhydrazy(DPPH·) and total reducing power. Based on Box-Behnken design,the optimal process parameters were obtained as:ethanol concentration 75%,extraction temperature 70 ℃,ratio of solid to liquid(g/mL)35,ultrasonic time 30 min. Under the optimal condition,the average yield of polyphenol was 1.1448%.Results indicated that the scavenging effects of the extract on ·OH,DPPH· were significant,the extraction also had slightly total reducing power. And within 0.1 ~0.6 mg/mL concentration,the scavenging effects of Sphallerocarpus gracilis stem leaves polyphenol on·OH,DPPH· was higher than BHT. The Sphallerocarpus gracilis stem leaves polyphenol with good antioxidant activity has the potential to be further developed and utilized as a natural antioxidant.

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