维生素对大肠杆菌Escherichia coli.JN8产L-色氨酸的影响

Effect of Vitamins on Production of L-Tryptophan in Escherichia coli.JN8

DOI：10.3969/j.issn.1673-1689.2013.09.004

中文关键词: 维生素 大肠杆菌Escherichia coti.JN8 L-色氨酸

英文关键词: vitamin Escherichia coli.JN8 L-tryptophan

基金项目:国家自然科学基金项目(21275066);江苏省科技创新与成果转化项目项目(重大科技支撑与自主创新专项BY2010117)

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中文摘要:

研究了9种维生素对大肠杆菌Escherichia coli.JN8生长及其发酵产L-色氨酸产量的影响,并对发酵培养基中的维生素浓度进行了优化。结果表明,低浓度肌醇和叶酸可显著促进大肠杆菌的生长及L-色氨酸的合成,叶酸和肌醇的最适添加量分别为0.000 2 mg/L和0.2 mg/L;较高浓度的生物素可以明显促进菌体生长及色氨酸的合成,其最适添加量为0.12 mg/L;较低浓度VB6的添加严重抑制色氨酸的合成,当其质量浓度增至0.8 mg/L时,菌体浓度和色氨酸的产量均较对照有显著的提高,分别提高11.8%和27.2%。添加VB5可略微提高色氨酸的产量,最优质量浓度为0.4 mg/L。添加VB1、VB2、VB3和VC虽然对菌体的生长均有较强的促进作用,但对于色氨酸合成却有不同程度的抑制作用。

英文摘要:

In this manuscript,the effect of 9 kinds of vitamins on overproduction of L-tryptophan and cell growth were studied and the concentration of vitamins in culture medium was also optimized. The results showed that the cell growth and the synthesis of L-tryptophan were both promoted strongly with the addition of low concentration of inositol and folic acid. Their optimum concentrations were 0.000 2 mg/L,0.2 mg/L,respectively. Adding higher concentration of biotin can significantly promote cell growth and the synthesis of L-tryptophan,the optimum dosage was0.12 mg/L. The synthesis of L-tryptophan was inhibited severely by adding lower concentration of VB6,when the optimal added concentration was 0.8 mg/L,cell concentration and L-tryptophan production have been significantly improved by 11.8% and 27.2% compared with the control,respectively. Adding VB5may slightly improve the yield of the L-tryptophan,the optimal concentration was 0.4 mg /L. Although,adding VB1,VB2,VB3and VC,cell growth was improved strongly,the synthesis of L-tryptophan were inhibited by different degrees.

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