BNP在不同缓冲体系中的血管舒张功能评价

Vasodilating Effect of BNP in Different Kind of Buffer Systems

DOI：10.3969/j.issn.1673-1689.2013.09.006

中文关键词: 人脑纳肽 柠檬酸/磷酸氢二钠缓冲溶液 离体血管灌流 注射剂处方

英文关键词: brain natriuretic peptide citric acid/Na2PO4buffer isolated vascular perfusion injectable prescription

基金项目:国家自然科学基金项目(81273437)

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中文摘要:

为了明确能使人脑纳肽(Brain Natriuretic Peptide)保持活性的适用缓冲溶液条件,采用常规离体血管灌流实验,观察人脑纳肽的不同缓冲溶液对家兔离体兔胸主动脉血管片段舒张功能的影响。研究发现,在以柠檬酸/磷酸氢二钠为基质的缓冲液条件下,BNP在10-8~10-5mol/L浓度区间对家兔血管舒张均呈良好的剂量依赖关系,而以醋酸/醋酸钠或磷酸氢二钾/磷酸二氢钾为基质的这两种缓冲液虽有一定的舒张效果,但没有剂量依赖关系。由此表明,在合适的pH条件下(4或5),柠檬酸/磷酸氢二钠缓冲液具有作为改进BNP注射剂处方的潜在价值。

英文摘要:

To investigate the vasodilating effect of BNP in different buffer solutions,rabbit thoracic aorta was rapidly dissected out and the effect of BNP on the tension of aortic rings was measured in an organ bath system. The results showed that BNP in Citric acid/Na2HPO4 buffer caused a concentration-dependent relaxation. However,BNP in NaAc/HAC or K2HPO4/KH2PO4 buffer has vasodilating effect in dose-independent manner. The results indicate that BNP in Citric acid/Na2HPO4 buffer could keep its vasodilating effect in a dose-dependent manner,implying that they may be effective in BNP formulation.

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