碱提杏鲍菇多糖PEAP-1的结构初探及形貌观察

Preliminary Structure Analysis and Structure Morphology Observation of Alkali-Extractable Polysaccharide PEAP-1 from Pleurotus Eryngii

DOI：10.3969/j.issn.1673-1689.2013.09.009

中文关键词: 碱提杏鲍菇多糖 分离纯化 结构初探 原子力显微镜 环境电镜扫描

英文关键词: Alkali-extractable polysaccharides from Pleurotus Eryngii purification Preliminary Structure Analysis AFM SEM

基金项目:国家自然科学基金项目(10874108);陕西省自然科学基础研究计划项目(SJ08A16)

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中文摘要:

采用碱提的方法从杏鲍菇中提取得到水溶性粗多糖PEAP,并经DEAE-52纤维素和SephadexG-150柱层析法分离纯化,获得一种新的杏鲍菇多糖组分PEAP-1。通过HPLC、GC、IR、刚果红实验、原子力显微镜(AFM),以及环境电镜扫描(SEM)分析方法,研究了PEAP-1的结构形貌。HPLC和GC结果表明,PEAP-1为均一多糖,相对分子质量为450 kDa,单糖基由葡萄糖和半乳糖组成,其摩尔比为16.9∶0.37。在红外光谱图中,832 cm-1和919 cm-1附近出峰,表明在PEAP-1中存在吡喃糖环结构。刚果红实验和AFM图像显示,PEAP-1的多分支结构中没有三股螺旋构型,单个分子呈无规则链状,直径在0.13~1.2 nm之间。同时,在SEM检测下PEAP-1呈片状、杆状和网状形貌。

英文摘要:

In this manuscript,the water-soluble crude polysaccharides PEAP was extracted from Pleurotus Eryngii(PE) by alkali solution and fractionated by DEAE-52 cellulose and Sephadex G-150,obtaining a novel polysaccharide composition termed PEAP-1. Furthermore,the structural characteristics of PEAP-1 was analyzed by HPLC,GC,IR,Congo red test,AFM and SEM methods.The results of HPLC and GC analysis indicated that PEAP-1 was a homogeneous polysaccharide whose average molecular weight is 450 kDa and monoaccharide is composed of Glu and Gal at a molar ratio of 16.9∶0.37. Moreover,the peak near 832 cm-1and 919 cm-1suggested the presence of pyranose ring in PEAP-1 from the infrared spectroscopy. The Congo red test and atomic force microscope(AFM) images showed that PEAP-1 has no triple-helix structure but with multi-branched frame,and its single molecular presents random coil chain with a diameter of 0.13~1.2 nm.Meanwhile,the flake,rods and mesh morphology of PEAP-1 was detected using SEM.

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