滇结香花对α葡萄糖苷酶的抑制活性

α-Glucosidase Inhibitory Activity of the Alabastrum of Edgeworthia gardneri(Wall.) Meissn

DOI：10.3969/j.issn.1673-1689.2013.09.012

中文关键词: 滇结香 α-葡萄糖苷酶 急性毒性实验 降血糖

英文关键词: Edgeworthia Meissn α-glucosidase acute toxicity studies antihyperglycaemic effect

基金项目:国家自然科学基金项目(31201020);江苏省自然科学基金项目(BK2010142)

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中文摘要:

对产于西藏的滇结香花选取极性大小不同的正己烷、石油醚、乙酸乙酯、甲醇、水依次进行提取;建立体外α葡萄糖苷酶抑制模型,对提取物进行活性评价;选择活性较高的组分进行小鼠急性毒性及对高血糖模型小鼠降糖作用评价。结果显示,滇结香花水提物(EMW,1 000 mg/kg)对酵母和大鼠来源的α-葡萄糖苷酶均具有较好的体外抑制活性,活性高于阳性对照阿卡波糖。EMW并未发现明显的毒副作用,EMW中高剂量(每千克体重用药量)200 mg/kg和300 mg/kg给药对小鼠高血糖模型具有良好的降血糖作用。研究表明,EMW具有较好的α-葡萄糖苷酶抑制及体内降糖活性。

英文摘要:

Edgeworthia gardneri(Wall.) Meissn,a medicinal herb endemic to the Tibetan region,is used to treat Diabetes. The aim of this study is to identify the active ingredients of different extracts from the alabastrum of E. gardneri by the yeast and the rat intestinal α-glucosidase inhibitory assay. The alabastrum of E. gardneri was partitioned sequentially with n-hexane,petroleum ether,ethyl acetate,methanol,and water. Its α-glucosidase inhibitory activity was assayed by the method of 96-microplates. Acute toxicity study of active components for inhibition of α-glucosidase was carried out in Kunming mice of both sexes. A mouse model of diabetic disease induced by alloxan was used to evaluation of hypoglycemic activity. The results showed that yeastα-glucosidase inhibitory activity of petroleum ether extracts,ethyl acetate extracts,methanol extracts and water extracts were higer than that of positive control Acarbose,but only water extracts of E.gardneri(EMW,1 000 mg/kg) had the rat intestinal α-glucosidase inhibitory activity. EMW treatment was not acutely toxic to the mice. EMW at doses of 200 mg/kg and 300 mg/kg significantly decreased blood glucose in diabetic mice. The results indicate that EMW has a better yeast and rat intestinal α-glucosidase inhibitory activity in vitro and possesses antihyperglycaemic effect significantly in vivo.

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