丁香提取物对人肝细胞H_2O_2氧化损伤的保护作用及其机制

Antioxidant Effects of Clove Polyphenols on RBL Cells Against Oxidative Damage

DOI：10.3969/j.issn.1673-1689.2013.09.018

中文关键词: 丁香提取物 抗氧化 人肝细胞 过氧化氢 机制

英文关键词: clove extracts antioxidant HL770 H2O2 mechanism

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中文摘要:

研究丁香提取物对由H2O2引起的人肝细胞HL770氧化损伤的保护作用及其机制。采用H2O2诱导建立细胞氧化损伤模型,通过测定细胞抗氧化酶活性、丙二醛、过氧亚硝基阴离子等指标,分析探讨丁香提取物对细胞损伤的保护作用。结果显示丁香水提取物和乙醇提取物可以剂量依赖性地提高细胞内的SOD、CAT和GSH-Px酶活性,降低氧化产物过氧亚硝基阴离子和丙二醛含量。表明丁香提取物可通过提高HL7702细胞内抗氧化酶活性和降低氧化产物的含量减轻细胞受到的氧化损伤。

英文摘要:

The objective of this study was to investigate the protective effects of clove extracts on HL770 caused by the damage of H2O2 and its protective mechanism.For this,oxidative damage was induced by H2O2.The influence of clove extracts on H2O2-induced HL770 injury was assessed by measuring the superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-Px)activities,the malondialdehyde(MDA)content and the ONOO-content.It was found that the addition of clove extracts(0.1,0.5,2,and 10 mg/L) into the HL770 cell suspensions prior to the exposure to 100 μmol/L of H2O2 resulted in increased protection of SOD,CAT and GSH-PX while reducing oxidation products of peroxynitrite MDA.From the above result,it is concluded that the clove extracts possess protective effects on HL770 cell injuries induced by H2O2,and this may be related to improve the activity of intracellular antioxidant enzymes of HL7702,reduce the content of oxidation products.

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