酶联免疫吸附法检测动物源性食品中氨苯砜残留

Determination of Dapsone Residue in Animal-Originated Food by Enzyme-Linked Immunosorbent Assay

DOI：10.3969/j.issn.1673-1689.2013.07.017

中文关键词: 酶联免疫吸附法 氨苯砜 动物源性食品

英文关键词: enzyme-linked immunosorbent assay(ELISA) dapsone animal-originated food

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中文摘要:

应用间接竞争酶联免疫吸附技术,建立了一种快速检测动物源性食品中氨苯砜残留的方法,并对其各项技术参数进行了评价。结果显示,该方法的半数抑制浓度IC50为0.369μg/L,对鸡肉、猪肉样本的检测限为0.2μg/kg,对鸡蛋、蜂蜜样本的检测限为2μg/kg,对牛奶样本的检测限为0.6μg/L;样本添加回收率为78.2%~104.0%,变异系数为5.2%~13.5%;对实际样本的检测结果与液相色谱-串联质谱法基本一致。该方法操灵敏度高、快速准确,适用于动物源性食品中氨苯砜残留的快速检测。

英文摘要:

A rapid detection method for dapsone residue in animal-originated food was established based on indirect competitive enzyme-linked immunosorbent assay.The results showed that the 50% inhibitory concentration(IC50) of the method was 0.369 μg/L.The detection limit for chicken and pork was 0.2 μg/kg,for egg and honey was 2 μg/kg,and for milk was 0.6 μg/L.Sample recoveries were 78.2%~104.0%,and the coefficients of variation were 5.2%~13.5%.Results of the method were in accordance with those of liquid chromatography-tandem mass spectrometry(LC-MS/ MS).This method has the advantage of being sensitive and accurate,and suitable for the rapid detection of dapsone residue in animal-originated food.

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