3-氯-1,2-丙二醇对睾丸间质瘤细胞R2C生物活性的影响

Effects of 3-Monochloropropane-1,2-Diol on the Cell Growth in Rat Leydig Tumor Cells R2C

DOI：10.3969/j.issn.1673-1689.2013.06.002

中文关键词: 3-氯-1,2-丙二醇 睾丸间质瘤细胞 孕酮

英文关键词: 3-Monochloropropane-1 2-diol leydig tumor cells progesterone

基金项目:国家“十二五”科技支撑计划项目(2012BAK01B00);国家自然科学基金项目(31201340)

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中文摘要:

为了探讨3-氯-1,2-丙二醇(3-Monochloropropane-1,2-diol,3-MCPD)对体外培养的大鼠睾丸间质瘤细胞R2C活性的影响,分别用0.5、1、2、4、6 mmol/L的3-MCPD刺激R2C细胞,利用MTT法得到3-MCPD对R2C细胞的IC25、IC50以及IC75。取以上3种不同浓度的3-MCPD作用细胞4 h和24 h,通过单细胞凝胶电泳法(Single Cell Gel Electrophoresis,SCGE)检测其作用4 h时对DNA损伤的程度;采用放射免疫法(Radio immunoassay,RIA)检测其作用4 h和24 h对孕酮合成的影响。结果显示:实验组与对照组相比,3-MCPD能抑制R2C细胞的增殖,其IC25、IC50和IC75分别为1.027、1.802、3.160 mmol/L;浓度为IC75的3-MCPD作用R2C细胞4 h后,对其DNA有损伤作用,且孕酮的合成量降低了16.0%;当3-MCPD各浓度组作用R2C细胞24 h后,与对照组相比孕酮合成量降低9.5%至61.2%。以上结果表明,3-MCPD能抑制R2C的活性,影响细胞孕酮的合成。

英文摘要:

This research aimed to investigate the effects of 3-Monochloropropane-1,2-diol(3 MCPD) on the cell growth in Rat Leydig tumor cells R2C.R2C cells were exposed to 0.5,1,2,4,6 mmol/L 3-Monochloropropane-1,2-diol for 48 h,different concentrations(IC25,IC50,IC75) of 3 MCPD were elected by MTT assay.Three specific concentrations of 3-MCPD were cultured with R2C cells for 4 h and 24 h,respectively.The damage of DNAs in R2C cells was investigated by single cell gel electrophoresis(SCGE).Progesterone level in the supernatant of cell culture medium was measured by radio immunoassay(RIA).Results indicated that 3-MCPD could inhibit cells growth,the IC25,IC50 and IC75 value were 1.027 mmol/L,1.802 mmol/L and 3.160 mmol/L,respectively.Compared with the control group,IC75 concentration of 3-MCPD incubated R2C cells for 4h,the damage of DNAs were obviously higher and the progesterone level were decreased 16.0%.In addition,three concentrations of 3-MCPD incubated R2C cells for 24 h,the progesterone level were obviously decreased from 9.5% to 61.2%.The study suggested that 3-MCPD could directly affect cell growth and the progesterone synthesis of R2C cells.

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