| 泡菜中乳杆菌的快速检出和乳杆菌质粒资源的初步调查
| Rapid Detection of Lactobacillus and a Preliminary Investigation of Lactobacillus Plasmids of Pickle
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| DOI：10.3969/j.issn.1673-1689.2013.06.014
| 中文关键词: 中国泡菜 乳杆菌 质粒 快速检出 PCR
| 英文关键词: Chinese pickle Lactobacillus plasmid rapid detection PCR
| 基金项目:国家自然科学基金项目(31170007)
| 作者
| 单位
| 杨四佳
| 成都医学院检验医学院,四川成都,610083
| 王颖
| 成都医学院检验医学院,四川成都,610083
| 李宇婷
| 成都医学院检验医学院,四川成都,610083
| 李晨
| 成都医学院检验医学院,四川成都,610083
| 张进
| 成都医学院检验医学院,四川成都,610083
| 刘锐
| 成都医学院检验医学院,四川成都,610083
| 潘渠
| 成都医学院基础医学院,四川成都,610083
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| 中文摘要:
| 乳杆菌(Lactobacillus)的质粒不仅决定了乳杆菌的某些性状,还应用于乳杆菌克隆表达载体的构建,是一种珍贵的遗传信息资源。为了调查中国泡菜中的乳杆菌质粒资源,采集了52份中国泡菜样品,采用乳杆菌PCR快速检出技术检出其中的乳杆菌,并用16s rDNA测序验证检出结果,然后提取检出乳杆菌菌株的质粒。结果检出1株清酒乳杆菌(Lactobacillus sakei)和18株植物乳杆菌(Lactobacillus plantarum),乳杆菌检出率为36.5%。检出含质粒的乳杆菌菌株11株,琼脂糖电泳显示有9种质粒谱型,乳杆菌质粒检出率为21.2%,质粒谱型检出率为17.3%。调查结果提示,中国泡菜中蕴藏丰富的乳杆菌质粒资源,值得进行更大规模的调查和研究。
| 英文摘要:
| Plasmids of Lactobacillus are precious genetic resources,which carried with some characteristics of Lactobacillus and used in construction of Lactobacillus cloning expression vectors.To investigate resources of Lactobacillus plasmids from Chinese pickle,we collected 52 samples of Chinese pickle,and isolated Lactobacillus strains from these samples using rapid-detection PCR technology which was designed by us.Isolated Lactobacillus strains were further confirmed by 16s rDNA sequencing.Finally,plasmids of isolated Lactobacillus strains were extracted.One Lactobacillus sakei strain and 18 Lactobacillus plantarum strains were detected,and the detection rate of Lactobacillus strain was 36.5%.Eleven Lactobacillus strains contained plasmids,and the detection rate was 21.2%.The results of agarose gel electrophoresis showed 9 band profiles which detection rate was 17.3%.The results of our investigation indicated Chinese pickle are rich in Lactobacillus plasmids,which need to be further study.
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