动物食品中去甲基睾丸酮的纳米增强酶联免疫分析方法

A Nanoparticle-Enhanced ELISA Against Nortestosteron in Animal-Original Food

DOI：10.3969/j.issn.1673-1689.2013.06.017

中文关键词: 去甲基睾酮 金纳米粒子 纳米增强酶联免疫分析

英文关键词: nortestosterone gold nanoparticle nanopaticle-enhanced ELISA

基金项目:教育部博士点新教师基金项目(20110093120003);江南大学大学生实践创新项目

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中文摘要:

作者通过辣根过氧化物酶标记抗体(HRP-IgG)蛋白分子固定在金纳米粒子(GNP)表面的方法,制备得到一种高活性的酶-金纳米复合物(HRP-IgG-GNP)。将该HRP-IgG-GNP应用于酶联免疫分析(ELISA)方法中,针对去甲基睾酮(NT),建立了一种高灵敏的纳米增强ELISA方法。该方法的检测灵敏度达到0.01 ng/mL,线性范围为0.03~10 ng/mL。与传统ELISA方法相比,该方法的灵敏度提高了10倍。该纳米增强ELISA方法有望开发高灵敏免疫分析方法中获得广泛应用。

英文摘要:

Conjugating enzyme with nanoparticles is an effective approach to enhance the characteristics of enzyme molecule in biosensor.A highly active enzyme-nanoparticle complex,HRP-IgG GNP,was prepared by immobilizing horseradish peroxidase labeled antibody(HRP-IgG) onto the surface of gold nanoparticle(GNP).The HRP-IgG GNP complex was applied to the enzyme-linked absorbent immunoassay(ELISA) and a highly sensitive nanopaticle-enhanced ELISA method was developed against nortestosterone(NT).The sensitivity of the novel method reached 0.01 ng/mL and the linear range was 0.03 ~10 ng/mL.the nanopaticle-enhanced ELISA method was 10 times more sensitive than the traditional ELISA method.The above method has promising applications for developing highly sensitive immunoassay methods.

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