无溶剂体系酶催化酯化反应合成1,3-甘油二酯的研究

Enzymatic Esterifiaction of 1,3-Diacylglycerol from Oleic Acid and Glycerol in a Solvent Free System

DOI：10.3969/j.issn.1673-1689.2013.04.004

中文关键词: 酯化反应 1,3-DAG Lipozyme RM IM 油酸 甘油

英文关键词: esterifiaction reaction 1 3-DAG Lipozyme RM IM oleic acid glycerol

基金项目:国家“十二五”科技支撑计划项目(2011BAD02B04)

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中文摘要:

在无溶剂体系中以油酸、甘油为原料,用专一性脂肪酶中合成1,3-DAG,实验发现真空脱水可以有效的增加油酸的转化率。在真空脱水的条件下,探讨了反应底物摩尔比、酶量和温度等因素对油酸的转化率和1,3-DAG质量分数的影响。结果表明:油酸与甘油摩尔比为2∶1时,酶量为6%,温度为65℃时1,3-DAG质量分数达到62%。

英文摘要:

1,3-diglyceride was esterified by oleic acid and glycerol using Lipozyme RM IM in a solvent free system.The effects of substrate molar ratio,reaction temperature and enzyme loading on the conversion rate of oleic acid and yield of 1,3-DAG were discussed in this paper.The results were as follows:molar ratio of oleic acid and glycerol 2 ∶1,reaction temperature 65 ℃,enzyme loading 6%,under these conditions,the 1,3-DAG yield was up to 62%.

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