胶质芽孢杆菌液体发酵产孢培养基的优化

Medium Optimization for Bacillus mucilaginous in Submerged Fermentation

DOI：10.3969/j.issn.1673-1689.2013.04.014

中文关键词: 胶质芽孢杆菌 芽孢产量 培养基 二次回归正交旋转组合设计 优化

英文关键词: Bacillus mucilaginous spore yield culture medium quadratic regression orthogonal rotation composite design optimization

基金项目:哈尔滨市科技局重点攻关项目(2011AA6BN071)

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中文摘要:

以提高胶质芽孢杆菌液体发酵芽孢产量为目的,以血球计数板观察计数芽孢量,对胶质芽孢杆菌液体发酵培养基组成进行优化研究。首先进行单因素试验,筛选最优的碳源、氮源、碳源浓度、氮源浓度、无机盐及其浓度,得到单因素最佳值;然后以二次回归正交旋转组合设计对得到的单因素最佳值进行优化,并进行验证试验。结果表明:最优产孢发酵培养基配方为每1000 mL培养基:乳糖21.57 g;复合氮源2.6 g,其中含蛋白胨与硫酸铵,二者质量比例为1∶3;硫酸锰0.21 g;磷酸氢二钾1.5 g;七水硫酸镁1.2 g;经二次回归正交旋转组合设计优化建立了回归模型,通过验证试验,血球计数板计数结果显示胶质芽孢杆菌芽孢产量可达3.44×109cfu/mL,实际芽孢量与回归模型预测值比为0.977,说明所得模型可靠。此优化培养基可以为工业生产胶质芽孢杆菌提供数据参考。

英文摘要:

This research was designed to optimize the culture medium for Bacillus mucilaginous in submerged fermentation.With spore yield as indicator,the test was divided into three phases.Firstly,based on the results of single factor experiments,the optimum carbon source,nitrogen source and metallic ion and their concentrations were screened and the optimal values of single factors were obtained;secondly,quadratic regression orthogonal rotation composite design was used to optimize the medium further and got the model;then,verification tests were carried out to verify the model.The results showed that the optimal culture medium were(per liter):lactose 21.57 g,combined nitrogen source 2.6 g(ratio of peptone to ammonium sulphate was 1∶3),MnSO4 0.21 g,K2HPO4 1.5 g,MgSO4.7H2O 1.2 g;cultured with the optimal medium,the spore number of Bacillus mucilaginous reached 3.44×10 9 cfu/mL;the ratio of actual spore yield to predicted value was 0.977,indicated that the model was reliable.The results could provide references for the producing of Bacillus mucilaginous.

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