桃果实总蛋白质双向电泳优化体系的建立

Optimization of Two-Dimensional Electrophoresis Conditions for Proteome Analysis of Peach Fruit

DOI：10.3969/j.issn.1673-1689.2013.03.004

中文关键词: 桃果实 总蛋白 双向电泳体系

英文关键词: Peach fruit protein extraction two-dimensional electrophoresis(2-DE)

基金项目:国家科技人员服务企业行动项目(2009GJC10010);江苏省研究生培养创新工程项目(CXZZ11-0658);江苏高校优势学科建设工程项目(PADA)

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中文摘要:

以"晖雨露"水蜜桃为试验材料,建立了一种适用于桃果实总蛋白质的双向电泳体系。最终试验确定采用酚抽法提取桃果实蛋白质,采用17 cm,pH 4~7的线性IPG胶条,800μg蛋白质上样,并用考马斯亮蓝G-250胶体考染,可以得到背景清晰、分辨率高的双向电泳图谱。对影响双向电泳图谱质量的因素进行了讨论分析。

英文摘要:

An effective two-dimensional electrophoresis system was developed to determine the total protein from "huiyulu" peach fruit in this study.The optimum conditions for total protein detection were listed as follows: phenol extraction,800 μg protein sample,17 cm IPG strip with pH 4-7,and finally detecting proteins with modified colloidal coomassie brilliant blue G-250 staining.With the optimum conditions,a high quality 2-DE map with low background was achieved.

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