枯草芽孢杆菌氨肽酶超滤提取技术

Research of the Ultrafiltration on the Bacillus subtilis Aminopeptidase Extraction

DOI：10.3969/j.issn.1673-1689.2013.03.010

中文关键词: 氨肽酶 超滤 浓缩 脱盐 膜通量 回收率

英文关键词: Aminopeptidase ultrafiltration concentration desalination flux recovery

基金项目:国家863计划项目(2011AA100905)

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中文摘要:

采用Ultra-flo超滤系统对经离心除菌、质量分数18%的(NH4)2SO4澄清的发酵液进行超滤浓缩和脱盐,为获得该过程中的高酶活回收率和膜通量,对其工艺条件进行了研究和优化;选用截留分子量为30 kDa的聚醚砜(PES)膜,操作压力为0.2～0.25 MPa,澄清发酵液初始pH值在5.5～6.5,浓缩倍数3倍;采用定时流加水的方式超滤脱盐,流加水体积控制为浓缩发酵液体积的10倍。在此优化条件下,超滤浓缩过程的酶活回收率达85%,超滤脱盐过程酶活回收率达79%,该过程可除去90%以上的盐,终浓缩倍数可控制为8～10倍;整个超滤过程的酶活回收率为67.15%,并且整个过程中膜通量维持在16 L/(m2.h)以上。

英文摘要:

The extraction parameters of aminopeptidase by Ultra-flo system from the fermentation broth is studied in this manuscript.The optimum conditions for high high enzyme activity recovery and membrane flux were listed as follows: 30 kDa MWCO polyether sulfone(PES) membrane,the operation pressure between 0.2 MPa and 0.25 MPa,the initial pH value 5.5～6.5 and the concentration ratiof 3 times;The elution is in the form of regular flow of water,and the elution volume is about 10 times of the concentration of the fermentation broth.By the optimun conditions,the enzyme activity recovery rate was achieved at 85% and 79% of the desalination process,and the salt in the concentration of the fermentation broth has been removed about 90%,and the final concentration factor can be controlled for 8～10times.The enzyme activity recovery rate can be 67.15% of the whole of the Ultrafiltration process,and the membrane flux can be maintained above 16 L/(m2·h) throughout the process.

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