蛹虫草Cordyceps militaris JN168产虫草素液态发酵条件的优化

Optimization of Fermentation Conditions for Cordycepin by Cordyceps militaris JN168

DOI：10.3969/j.issn.1673-1689.2013.02.005

中文关键词: 蛹虫草 虫草素 发酵 优化

英文关键词: Cordyceps militaris cord ycepin fermentation optimization

基金项目:国家863计划项目(2010AA101501);江苏省科技创新与成果转化(重大科技支撑与自主创新专项引导资金)项目(BY2010117)

摘要点击次数: 255

全文下载次数: 879

中文摘要:

利用单因素筛选和响应面法对蛹虫草Cordyceps militaris JN168产虫草素的液态发酵培养基进行优化,以确定蛹虫草产虫草素的最佳发酵培养基配方。结果表明,蛹虫草产虫草素的最佳碳源为葡萄糖,最适质量浓度为40 g/L;最佳氮源为牛肉膏,最适质量浓度15 g/L;加入的无机盐及其添加量分别为MgSO40.76 g/L,K2HPO40.63 g/L,CaCl20.66 g/L,Na2HPO40.67 g/L。优化后发酵液中虫草素质量浓度达到633.47 mg/L,是优化前的6倍。

英文摘要:

The components for cordycepin production by Cordyceps militaris JN168 were optimized with single factor experiment and response surface methodology. The purpose of this study is to determine the best fermentation medium for cordycepin production by Cordyceps militaris JN168. Results showed that glucose and beef extract are the appropriate carbon and nitrogen sources with an optimal concentration of 40 g/L and 15 g/L,respectively. And adding inorganic salt is MgSO 4 , K 2 HPO 4 ,CaCl 2 and Na 2 HPO 4 with an optimal concentration of 0.76 g/L,0.63 g/L,0.66 g/L and 0.67 g/L respectively. The cordycepin concentration in the fermentation broth after optimization is 633.47 mg/L,which is 6 times before the optimization.

查看全文 查看/发表评论 下载PDF阅读器