蜂蜜中细菌的筛选、鉴定及其抑菌作用

Screening and Identification and Study on Antimicrobial Activity of Bacterial Isolates from Honey

DOI：10.3969/j.issn.1673-1689.2013.02.007

中文关键词: 蜂蜜 芽孢杆菌 鉴定 抑菌作用

英文关键词: honey Bacillus identification antimicrobial activity

基金项目:国家863计划项目(2010AA101501);重大科技支撑与自主创新专项引导资金项目(BY2010117);江苏高校优势学科建设工程项目

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中文摘要:

从4种蜂蜜中分离得到8株芽孢杆菌,通过生理生化实验及形态学观察,结合16S rRNA基因序列分析对这些菌株进行了鉴定。初步研究了8株分离菌对大肠杆菌(Escherichia coli)、痢疾杆菌(Shigella dysenteriae S7NaCl)、粘质沙雷氏菌(Serratia marcescens SYBCT02)的抑菌作用。通过对这些分离菌的抑菌性质的研究,发现有些菌株是通过竞争作用抑制其他菌落的生长,还有些菌株是通过其代谢过程产生的抑菌物质来抑制其他菌落的生长。

英文摘要:

Eight Bacillus strains were screened out successfully from four honey samples.Theses strains were identified by colony morphology,physiological and biochemical characteristics,and 16S rRNA gene sequence analisis.Eight isolates exhibited antimicrobial activity against Escherichia coli,Shigella dysenteriae S7NaCl and Serratia marcescens SYBCT02.Based on the study of antimicrobial activity by the isolates of bacteria,it was found that some strains might inhibit other colonies growth through competitive,and some strains might inhibit other colonies growth by its metabolism produced inhibitory substances.

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