MAE-GC/MS法测定食品中B(α)P

MAE-GC/MS Determination Foods B (α) P Content

DOI：10.3969/j.issn.1673-1689.2014.12.016

中文关键词: 食品 微波辅助萃取技术 气相色谱/质谱

英文关键词: Food, MAE(microwave-assisted extraction technology), gas chromatography/mass spectrometry(GC/MS)

基金项目:

摘要点击次数: 169

全文下载次数: 533

中文摘要:

建立了测定食品中苯并芘(B(α)P)含量的MAE-GC/MS法。样品经微波辅助萃取技术前处理, DB-5MS弹性石英毛细管柱(30 m×0.25 mm×0.25 μm)分离, 以B(α)P m/z＝252为选择离子, 串连四极杆选择离子检测模式检测, 再以9-苯基蒽为内标, 采用内标法定量。结果显示, B(α)P的检出限为0.01 μg/kg, 标准曲线线性范围为0~10 μg/mL, 相关系数为1.000, 方法回收率达97%~103％; 精密度良好：日内RSD＜0.5%, 日间RSD＜1%; 准确度良好：以阴性样品为基底物质, 在0.1 mL甲醇苯并芘成分分析国家标准物质(GBW08702)的加标水平上, 测定值在其标准值范围内。表明：该法前处理简单, 回收率高, 检出限、线性范围、准确度、精密度结果均满足要求, 可用于测定食品中的B(α)P含量。

英文摘要:

The aim of this study is to develop MAE-GC/MS method to determine the Benzo(α) pyrene (B(α)P) content. For this, the sample was pre-treatment by MAE(microwave-assisted extraction technology)and then separate by, DB-5MS fused silica capillary column(30 m×0.25 mm×0.25 μm) with B(α)P m/z=252 asthe selected ion, the detection is the series four quadrupole selected ion monitoring(SIM) mode with 9-phenyl anthracene as internal standard. A series of results were achieved： B(α)P detection limit was 0.01 μg/kg and the standard curve was linear at the range of 0~10 μg/mL. The correlation coefficient was 1.000, with the recoveries of 97%~103% and good precision: Days RSD/%

查看全文 查看/发表评论 下载PDF阅读器