原生贡寮山药萃取物抗氧化成分及抗氧化活性的研究

Antioxidant Abilities and Compounds of Extractions from One Kind of Taiwanese Yam (Dioscores alata)

DOI：10.3969/j.issn.1673-1689.2014.10.015

中文关键词: 原生贡寮山药 山药皮 山药肉 抗氧化 胞内活性氧分析 HPLC

英文关键词: Dioscores alata,peal,meat,antioxidant activities,ROS,HPLC

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中文摘要:

探讨了干燥、萃取方式对原生贡寮山药（Dioscores alata）抗氧化成分以及抗氧化活性的影响。抗氧化成分分析了总多酚质量分数；体外抗氧化的测定方法包括DPPH自由基清除能力与还原力。结果表明，山药皮冷冻干燥后经过乙醇萃取所得萃取物的总多酚质量分数以及抗氧化能力最高。抗氧化能力及总多酚质量分数以冷冻干燥优于热风干燥，萃取方式以50%体积分数乙醇溶液最优，山药皮优于山药肉。HPLC分析萃取物中主要多酚成分为绿原酸。同时对体外抗氧化活性以及总多酚含量较高的原生贡寮山药的乙醇萃取物进行抑制体内细胞ROS产生测试，结果显示各个样品均可以显著降低由LPS所诱导细胞ROS的产生，抑制效果跟抗氧化能力结果一致。

英文摘要:

The effects of drying and extraction processes on the antioxidant compounds and antioxidant activities of one kind of yam（Dioscorea alata）， were investigated. The antioxidant compounds analyzed the phenolic content. The antioxidant measurements included a ，a-diphenyl-b-pricryl-hydrazyl（DPPH） radical-scavenging activity and reducing power . The 50% ethanolic extracts from the freeze-dried peel had the most antioxidant compouds and the highest antioxidant activities. Generally speaking，freeze-dried yam maintained higher antioxidant activities than hot air-dried ，the peel had more than the meat，Among three extraction methods，50% ethanolic extraction was the best.By HPLC analysis showed that the major polyphenols of the extraction was chlorogenic acid. Meanwhile tested the 50% ethanolic extracts on the inhibition of the formation of ROS，we got the same resulet as before.

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