樟芝提取物Fr.2组分对PDGF-BB活化CFSC-8B的抑制作用

Inhibitory Effects of Extract Fraction 2 from Antrodia cinnamomea on the Activation of CFSC-8B Cells Induced by PDGF-BB

DOI：10.3969/j.issn.1673-1689.2014.09.004

中文关键词: 血小板衍生生长因子-BB 肝星状细胞 肝纤维化 MAPK信号通路 樟芝

英文关键词: platelet-derived growth factor-BB, Hepatic stellate cells, Liver fibrosis, MAPK signaling, Antrodia cinnamomea

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中文摘要:

为了探讨樟芝分离组分Fr.2对血小板衍生生长因子-BB（PDGF-BB）刺激的大鼠肝星状细胞（HSCs）的作用，建立PDGF-BB活化的CFSC-8B细胞模型，并对细胞增殖、迁移、细胞外基质表达及机制进行了研究。结果表明，10 ng/mL PDGF-BB作用48 h可诱导CFSC-8B细胞增殖；Fr.2的IC50值为56.46 μg/mL；25 μg/mL Fr.2显著抑制PDGF-BB刺激CFSC-8B细胞的增殖及其迁移，下调α-SMA、Collagen I（Col 1）、Collagen Ⅲ（Col 3）和Fibronectin（Fn）mRNA表达水平，并下调p-ERK、p-JNK、p-P38蛋白质表达。表明樟芝Fr.2可抑制PDGF-BB诱导的细胞增殖、迁移及细胞外基质的表达，其机制可能与抑制MAPK信号转导通路有关。

英文摘要:

This research aimed to investigate the effects of extract fraction 2(Fr.2) from Antrodia cinnamomea on the rat hepatic stellate cells(HSCs). A cultured rat HSC line CFSC-8B was stimulated by PDGF-BB in the presence or absence of Fr.2. Cell proliferation, cell migration, extracellular matrix(ECM) production and anti-fibrotic mechanism were studied. We found that CFSC-8B cell proliferation could be induced by 10 ng/mL PDGF-BB for 48 h. The IC50 value of Fr.2 was 56.46 μg/mL. Fr.2(25 μg/mL) significantly inhibited CFSC-8B cell proliferation and migration induced by PDGF-BB. Fr.2 also decreased α-SMA, Col 1, Col 3 and Fn mRNA expression. Mechanistically, Fr.2 negatively regulated p-ERK, p-JNK and p-P38 expression. Therefore, we provide evidence to demonstrate Fr.2 from A. cinnamomea inhibited HSCs proliferation, migration and ECM production induced by PDGF-BB, which in part, through negatively regulates MAPK signaling.

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