樱桃叶黄酮的体外抗氧化活性

Antioxidant Activity of Flavonoids from Cherry Leaf in Vitro

DOI：10.3969/j.issn.1673-1689.2014.09.012

中文关键词: 樱桃叶 黄酮 抗氧化活性 体外

英文关键词: cherry leaves, flavonoids, antioxidant activity, in vitro

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中文摘要:

考察樱桃叶黄酮的体外抗氧化活性，为樱桃叶黄酮在医药、食品方面的应用提供理论依据。从樱桃叶中提取黄酮，体外检测樱桃叶黄酮对羟自由基（·OH）和超氧阴离子自由基（O2-·）的清除作用，以及对H2O2诱发小鼠红细胞氧化溶血和肝脂质过氧化反应的抑制作用。结果显示，樱桃叶黄酮对·OH和O2-·具有清除作用，且呈现质量浓度依赖性，30 μg/mL的黄酮溶液对·OH和O2-·的清除率分别达到85.67%和72.45％，并且对·OH的清除能力优于维生素C；樱桃叶黄酮对肝匀浆脂质过氧化反应和过氧化氢（H2O2）诱发红细胞氧化溶血具有抑制作用，当终质量浓度为30 μg/mL时，抑制率分别为79.82%和75.54%。表明樱桃叶黄酮具有质量浓度依赖性的体外抗氧化活性。

英文摘要:

This study was undertaken to extract flavonoids from cherry leaves and to investigate the anti-oxidative activity in vitro. Total flavonoids were extracted from cherry leaves. The antioxidant activities of the flavonoids were evaluated by the scavenging power on free radicals ·OH and O2-· and inhibiting effects on erythrocytes hemolysis and lipid peroxidation of hepatic homogenate in vitro system. The flavonoids showed concentration-dependent scavenging power on ·OH and O2-·. The scavenging rates for ·OH and O2-·of 30 μg/mL flavonoids solution were 85.67% and 72.45% respectively, and the scavenging capacity for ·OH was stronger than Vitamin C. Cherry leaves flavonoids also showed inhibition on lipid peroxidation of hepatic homogenate and on hydrogen peroxide-induced erythrocyte hemolysis in a concentration-dependent manner. The inhibition rates were 79.82% and 75.54% in final concentration 30 μg/mL. Cherry leaves flavonoids have concentration-dependent antioxidant activity in vitro, which can be used as natural plant antioxidants or food additives for the development and utilization.

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