响应面优化超声辅助提取莲子心总生物碱工艺

Optimization of Ultrasonic-Assisted Extraction of Total Alkaloids from Plumula Nelumbinis by Response Surface Methodology

DOI：10.3969/j.issn.1673-1689.2014.07.012

中文关键词: 莲子心 总生物碱 超声辅助提取 响应面优化

英文关键词: plumula nelumbinis, total alkaloids, ultrasonic-assisted extraction, response surface methodology

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中文摘要:

利用响应面分析法对超声辅助提取莲子心中总生物碱的工艺条件进行优化。在单因素实验、Plackett-Burman实验设计和最陡爬坡实验的基础上，以莲子心总生物碱提取量为响应值，利用效应响应面法研究各因素及其交互作用对莲子心总生物碱提取量的影响，优选莲子心中总生物碱的提取工艺。作者采用非线性数学模型拟合的方式，使自变量和因变量的关系扩展到曲面，从而使实验数据更加精密。实验优化得出超声辅助提取莲子心总生物碱的最佳工艺条件为：料液质量体积比1 g∶40.4 mL，乙醇体积分数64.4%，超声提取时间40 min，模型预测可达到128.695 mg/g。在上述条件下，莲子心总生物碱的提取率可以达到128.673 mg/g，与模型预测值基本一致。

英文摘要:

The optimization of the ultrasonic-assisted extraction of total alkaloids from Plumula Nelumbinis by response surface methodology was studied. Based on the single-factor test analysis, the Plackett-Burman experimental design and steepest ascent design, while take extracted amount of total alkaloids as the response value, response surface method was applyed to analyze the influence of various factors and their interaction on the extraction of total alkaloids, select an optimized progress to extract total alkaloids from Plumula Nelumbinis. In this study, nonlinear mathematical model fitting way make independent variables and the dependent variable relationship extend to curved surface, so that the experimental data become more precise. The experiments obtain an optimal ultrasound-assisted extraction of total alkaloids from Plumula Nelumbinis progress conditions: solid-liquid ratio as 1∶40.4; volume fraction of ethanol as 64.4 %; ultrasound extraction time as 40 min; model predict its productive yield can reach 128.695 mg/g. Under these conditions, extracted amount of total alkaloids from Plumula Nelumbinis can reach 128.673 mg/g, which is consistent with the predicted values.

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