多种致病菌同步检测方法的研究进展

Research Advance Study of the Synchronous Detection of Multiplex Foodborne Pathogens

DOI：10.3969/j.issn.1673-1689.2012.05.001

中文关键词: 多种致病菌 同步检测

英文关键词: detection targets multiplex foodborne pathogens synchronous detection

基金项目:国家自然科学基金项目(20806033);2010年度公益性行业(农业)科研专项项目(201003008-08)

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中文摘要:

概述了致病菌检测的对象,包括细菌菌群的形态及细菌本身、细菌的DNA、细菌的代谢物;同时介绍了可同步检测多种致病菌的方法,主要有电化学DNA传感器、适配体电化学传感器、免疫传感器检测技术、多重PCR检测、表面增强拉曼光谱检测;最后总结检测方法未来的发展方向。

英文摘要:

In this review,the detection targets such as bacteria colonies,bacteria cell i,DNA and bacteria metabolic products are firstly introduced.Then thedetection methods which can synchronously indentify Multiplex Food-borne Pathogens electrochemical DNA biosensor,Aptamer biosensor,Electrochemical immunosensor,Surface plasmon resonance biosensor and Multiplex PCR were summarized.Based on the above progress,the the challenge and perspective of detection method were put forward.

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