酿酒酵母抗氧化相关基因突变体对黄曲霉毒素B_1的清除作用

Detoxification of Aflatoxin B1 by Saccharomyces cerevisiae Mutants of Anti-Oxidative Relating Genes

DOI：10.3969/j.issn.1673-1689.2012.05.004

中文关键词: 黄曲霉毒素 酿酒酵母 抗氧化基因 生物脱毒

英文关键词: aflatoxin Saccharomyces cerevisiae anti-oxidative gene detoxification

基金项目:国家自然科学基金项目(No.30870056)

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中文摘要:

黄曲霉毒素(AF)是粮食作物和饲料原料中容易污染的一种强毒性和强致癌性物质,酿酒酵母具有毒素清除功能。利用HPLC分析了酿酒酵母野生菌BY4742及三株关键的抗氧化相关基因缺失菌zwf1Δ、sod2Δ、glr1Δ对黄曲霉毒素B1的清除能力。结果表明,在PBS缓冲液中存活和死亡的细胞对AFB1的清除率分别为74%～76%和71%～73%,说明酵母细胞对AFB1的清除以生物吸附作用为主。在培养基中,3种突变菌活细胞对AFB1的清除率发生不同程度的降低,其中glr1Δ的AFB1清除能力下降最明显,其次是sod2Δ,而zwf1Δ下降最少,说明这些关键的抗氧化基因的缺失会影响细胞在生长状态下对AFB1的清除作用。

英文摘要:

Aflatoxins are a group of mycotoxins with strong mutagenic and carcinogenic properties.Various commodities including crop and feed materials are easy to be contaminated with aflatoxin.Saccharomyces cerevisiae have been reported to bind or degrade aflatoxin.Here,detoxification of aflatoxin B1(AFB1) by wild-type strain of S.cerevisiae(BY4742) and three mutants of anti-oxidative relating genes(zwf1Δ,sod2Δ and glr1Δ) were determined by HPLC.In PBS buffer,AFB1 binding abilities of viable and dead cells were 74%-76% and 71%-73%,respectively,indicating AFB1 was removed by yeast cells mainly through cell adsorption.In YPD medium,clearance of AFB1 by three mutant viable cells reduced,while that by wild-type BY4742 remaining high.AFB1 binding ability of glr1Δ decreased most seriously,then was that of sod2Δ and zwf1Δ.Thus,the deletion of critical anti-oxidative relating genes would decrease the AFB1 binding ability of S.cerevisiae growing cells.

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