抑制剂对副溶血性弧菌运动性的影响

Effect of Inhibitors on the Motilities of Vibrio parahaemolyticus

DOI：10.3969/j.issn.1673-1689.2014.05.005

中文关键词: 副溶血性弧菌 运动性 泳动 群集 蹭行

英文关键词: Vibrio parahaemolyticus, motility, swimming, swarming, twitching

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中文摘要:

通过添加不同抑制剂（EDTA、亚硝酸钠及氯化镁）观察副溶血性弧菌在泳动、群集和蹭行平板上迁移形成的可见圈大小来研究这些抑制剂对其运动性的影响。结果表明：EDTA、亚硝酸钠、氯化镁和茶多酚均可抑制副溶血性弧菌的泳动、群集及蹭行，培养基中分别含0.20 mmol/L的EDTA、50 mg/L亚硝酸钠、15.0 g/L氯化镁时，能明显抑制副溶血性弧菌的三种运动行为。因此，三种抑制剂对副溶血性弧菌的运动性有明显的影响，可作为控制副溶血弧菌污染的有效手段。

英文摘要:

The effects of three inhibitors(ethylenediaminetetraacetic acid (EDTA), sodium nitrite and magnesium chloride) on the bacterial motilities of Vibrio parahaemolyticus were investigated by testing the flagellum-mediated swimming, flagellum-mediated swarming, and type IV pilus-mediated twitching on different plates. The motilities were evaluated by measuring the size of colony in the culture adding with EDTA, sodium nitrite and MgCl2, respectively. The results showed that the three types of the motilities could be inhibited by EDTA, sodium nitrite, and magnesium chloride, and the effective concentrations of the three inhibitors in plates were 0.10 mmol/L, 50 mg/L, and 15.0 g/L, respectively. The results indicated that EDTA, sodium nitrite, and magnesium chloride have the strong influence on the motilities of Vibrio parahaemolyticus, so the three inhibitors can effective control the contamination of Vibrio parahaemolyticus.

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