油菜蜂花粉中核苷类成分的分离及鉴定

Separation and Identification the Nucleoside Components From Rape Bee Pollen

DOI：10.3969/j.issn.1673-1689.2014.04.010

中文关键词: 油菜蜂花粉 分离纯化 核苷 结构鉴定

英文关键词: Rape bee pollen, separation and purification, nucleoside, structure identification

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中文摘要:

经粉碎后的油菜蜂花粉用去离子水提取，经大孔树脂AB-8色谱柱粗分离，再用MCI-GEL色谱柱和ODS-B色谱柱分离，TLC薄层法检测纯度，最终得到3个化合物。经1H-NMR，13C-NMR和135DEPT-NMR鉴定分析，3个化合物分别为：鸟嘌呤核苷、腺嘌呤核苷、胞腺嘧啶脱氧核苷。这3种核苷类化合物是首次从油菜蜂花粉中分离、鉴定的核苷类物质。

英文摘要:

Rape bee pollen is crushing by grinder, then extraction with deionized water, take appropriate amount of the extract, through the macroporous resin column of AB-8 to crude separation, and then isolated by the MCI-GEL column and the ODS-B column , and at last I get three pure compounds. These three compounds are Being analyzed by 1H-NMR, 13C-NMR and 135DEPT-NMR, These three compounds were: guanosine, adenosine, deoxycytidine. These three kinds of nucleosides is the first time direct purification and structural identification from rape bee pollen.

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