辣椒制品中罗丹明B的基质固相分散提取条件优化

Matrix Solid-Phase Dispersion Extraction Conditions of Rhodamine B from Chili Products

DOI：10.3969/j.issn.1673-1689.2014.03.008

中文关键词: 罗丹明B 正交试验 基质固相分散萃取

英文关键词: rhodamine B orthogonal experiment matrix solid-phase dispersion extraction

基金项目:国家“十二五”科技支撑计划项目(2012BAK25B01、2012BAK08B06-1)

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中文摘要:

采用改进的基质固相分散提取技术提取辣椒制品中的罗丹明B,对提取工艺进行优化。选择分散剂种类、分散剂与样品质量比、洗脱剂组成及洗脱剂用量作为研究因素,以提取液中罗丹明B的含量为考察指标,运用L16(45)正交试验优选出了辣椒面中提取罗丹明B的最优工艺条件:分散剂硅胶,分散剂与样品质量比例1∶1;洗脱剂为质量分数80%乙腈水溶液,洗脱剂用量20 mL。辣椒油中提取罗丹明B最优工艺条件是:分散剂中性氧化铝,分散剂与样品质量比例2∶1;洗脱剂为体积分数80%甲醇水溶液,洗脱剂用量20 mL。辣椒酱中提取罗丹明B最优工艺条件是:分散剂中性氧化铝,分散剂与样品质量比例3∶1;洗脱剂为质量分数80%乙腈水溶液,洗脱剂用量5 mL。该提取方法萃取工艺简单,操作时间短,所需有机溶剂种类少,属环境友好型萃取方式。

英文摘要:

with the Rhodamine B content in extract as index, the optimum parameters,including dispersant type,the ratio of dispersing agent and sample,composition of the eluent and elute volume,of extracting Rhodamine B from chili were investigated by using orthogonal design L16(45).The optimum conditions for extracting Rhodamine B from chili powder are as follows:dispersant type is silica gel,the ratio of dispersing agent and sample is 1∶1,composition of the eluent is 80% of acetonitrile,elute volume is 20 mL. The optimum conditions for extracting Rhodamine B from pepper oil are as follows:dispersant type is neutral aluminum oxide,the ratio of dispersing agent and sample is 2 ∶1,composition of the eluent is 80% of methanol,elute volume is 20 mL. The optimum conditions for extracting Rhodamine B from chili paste are as follows:dispersant type is neutral aluminum oxide,the ratio of dispersing agent and sample is 3∶1,composition of the eluent is80% of acetonitrile,elute volume is 5 mL. The extraction method is simple,convenient,less material consumption and less organic solvent.

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