不同形态的果糖对SD大鼠炎症因子及内脏组织的影响

Effects of Free and Bound Fructose on Inflammatory Factors and Viscera Histopathology in Sprague-Dawley Rat

DOI：10.3969/j.issn.1673-1689.2014.02.008

中文关键词: 游离态果糖 结合态果糖 炎症因子 肝脏 胰脏

英文关键词: free fructose bound fructose inflammation factor liver pancreas

基金项目:国家自然科学基金重点项目(31230057)

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中文摘要:

探讨长期喂养游离态果糖和结合态果糖对大鼠炎症因子及内脏组织病理的影响。雄性SD大鼠40只,随机分为4个实验组及1个对照组,经过11 w喂养后,处死解剖,ELISA法检测血清及肝脏单核细胞趋化蛋白-1(MCP-1)与肿瘤坏死因子-α(TNF-α)的质量分数,苏木素-伊红(HE)染色法观察肝脏、胰脏组织病理学变化。结果显示,与对照组相比,含果糖实验组大鼠血清及肝脏MCP-1与TNF-α的质量分数均高于对照,其中游离态果糖组(F/G)血清MCP-1与TNF-α质量分数显著低于结合态果糖组(SU)(P

英文摘要:

Long-term effects of free and bound fructose on inflammatory factors and viscera histopathology in Sprague-Dawley rat were investigated. Male rats were randomized into four experimental groups and a control group. Each group was fed a diet containing 68% of total calories as different composition of carbohydrate for 11 weeks. At the end of the experiment,rats were sacrificed by anesthesia and were autopsied. Serum monocyte chemoattractant protein-1(MCP-1) and tumor necrosis factor-α(TNF-α) were detected by ELISA method. The pathological changes of liver and pancreas were directed with hematoxylin & eosin(H&E) staining. The results showed that the levels of serum and liver TNF-α and MCP-1 in fructose-fed rats were higher than the control.Moreover,the bound fructose(i.e. sucrose) showed higher serum MCP-1 than the free fructose(F/G)(P

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