麦冬多糖的修饰及其抗氧化活性与空间结构的研究

Study on Antioxidant Activity and Spatial Structure of Ophiopogon japonicus Polysaccharide with Modified

DOI：10.3969/j.issn.1673-1689.2014.01.005

中文关键词: 麦冬 多糖 化学修饰 抗氧化 刚果红

英文关键词: Ophiopogon Japonicus polysaccharide chemical modifed antioxidant activities Congo red

基金项目:国家自然科学基金项目(10874108);陕西省自然科学基金项目(SJ08A16)

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中文摘要:

研究热水浸提和超声波辅助法提取的两种麦冬多糖以及它们修饰之后的抗氧化活性及三股螺旋结构。采用硫酸化、磷酸化、羧甲基化对多糖进行修饰,并用红外光谱表征,进行抗氧化性的测定,利用刚果红实验进行三股螺旋结构的检测。结果表明:各种多糖都有一定的体外抗氧化活性,其中超声提取的效果较优于热水浸提的。这几种多糖对DPPH和超养阴离子自由基的清除能力较强,但对羟基自由基的清除能力较弱,且经过修饰之后的多糖有一定程度的三股螺旋结构,而未经修饰的没有三股螺旋结构。

英文摘要:

In this manuscript, the antioxidant activities in vitro and triple helix structure of the different chemical modified on Ophiopogon Japonicus polysaccharide,which extracted by hot water and ultra-sound were investigated. They were modified by suflation,phosphorylation and carboxymethylation,and characterized them by IR. Finally assay their antioxidant activities and determined the triple helix structure with Congo red experiment. Results showed that these polysaccharides all have a certain degree of antioxidant activities in vitro,which the effect of ultrasound extraction is better than that of the hot waterextraction. Moreover they significantly decrease the DPPH and superoxide anion radicals,but they had little scavenging effect on the hydroxyl radicals. After modified,the polysaccharides show the triple helix structure,however the unmodified polysaccharides were not express the triple helix structure.

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