| 聚丙烯膜固定化转谷氨酰胺酶的条件优化
| Optimization of Immobilization of Transglutaminase on Polypropylene Membrane
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| DOI：10.3969/j.issn.1673-1689.2014.01.012
| 中文关键词: 转谷氨酰胺酶 紫外接枝 固定化
| 英文关键词: transglutaminase ultraviolet grafting immobilized
| 基金项目:黑龙江省高等学校青年学术骨干支持计划项目(1253G048)
| 作者
| 单位
| 钱镭
| 黑龙江东方学院食品与环境工程学部,黑龙江哈尔滨150086
哈尔滨商业大学食品工程学院,黑龙江哈尔滨150076
| 任德财
| 黑龙江东方学院食品与环境工程学部,黑龙江哈尔滨,150086
| 韩春然
| 哈尔滨商业大学食品工程学院,黑龙江哈尔滨,150076
| 张娜
| 哈尔滨商业大学食品工程学院,黑龙江哈尔滨,150076
| 刘颖
| 哈尔滨商业大学食品工程学院,黑龙江哈尔滨,150076
| 马永强
| 哈尔滨商业大学食品工程学院,黑龙江哈尔滨,150076
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| 中文摘要:
| 研究了转谷氨酰胺酶在聚丙烯微孔膜上固定化的影响因素。采用响应面分析方法对紫外接枝反应条件进行优化,确定了最佳的膜固定化方法。结果表明,照射距离为10.1 cm,单体浓度为18.85%,二次照射时间为21.4 min时,接枝率可达34.62%。转谷氨酰胺酶固定化的条件为:己二胺质量分数为20%,胺烷基化时间90 min,胺烷基化温度50℃;戊二醛浓度2%,戊二醛作用时间40 min;酶晶体溶液质量浓度15 mg/mL,4℃条件下固定化时间为24 h。
| 英文摘要:
| The factors of chemical immobilization of transglutaminase on polypropylene membrane were studied. The conditions of graft polymerization were optimized by using response surface methodology,and the optimal conditions of immobilization were determined by experiments. The results indicate that the optimum graft polymerization conditions as followings:distance of ultraviolet was 10.1 cm,consistency of methyl-methacrylate was 18.85%,and the time of ultraviolet was 21.4 minutes. The best grafting degree of the membrane was 34.62%. The conditions of immobilization was:consistency of hexamethylendiamine was 20%,at 50 ℃ for 90 min and consistency of glutaraldehyde was 2%,at 30 ℃ for 40 minutes,consistency of enzyme was 15 mg/mL,at 4 ℃ for 24 hours.
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