电场对脂肪酶二级结构及其活性的影响

Study on the Effect of Electric Field on the Secondary Structure and Activity of Lipase

DOI：10.3969/j.issn.1673-1689.2015.12.004

中文关键词: 主题词： 电场 脂肪酶 红外光谱 二级结构 酶活性

英文关键词: electric field,lipase,FTIR,secondary structure,activity

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中文摘要:

脂肪酶被不同强度的电场处理5 min，用红外光谱法研究电场对其二级结构的影响，并测定酶活性的变化。实验结果显示：在1.0～6.0 kV/cm范围，电场作用对脂肪酶二级结构单元相对含量的影响程度不同。与对照组相比，处理组的α－螺旋和β－折叠含量普遍减少，减少幅度分别为4.9%～10.9%和3.2%～22.6%；β－转角和无规卷曲含量普遍增加，增加幅度分别为12.5%～37.7%和3.1%～24%。并且各种结构的含量在3.0 kV/cm电场强度条件下变化最为明显。经电场处理后脂肪酶活性显著增加，这与β－转角和无规卷曲含量的增加有一定的相关性。

英文摘要:

The changes in the secondary structures and activity of lipase after 5 min treatment using electric field with different strength were studied by FTIR（Fourier-transformation infrared spectroscopy）. The effect of electric field on the relative contents of the lipase secondary structures，i.e. α-helix，β-sheet，β-turn and random coil，varied within the range of 1.0 kV/cm to 6.0 kV/cm. Compared with the control groups，the relative contents of α-helix and β-sheet both decreased，namely，4.9%~10.9% for α-helix and 3.2%~22.6% for β-sheet respectively. The relative contents of β-turn and random coil increased instead，namely，12.5%~37.7% for β-turn and 3.1%~24% for random coil respectively. The most significant change of the relative contents for different secondary structure was observed at the 3.0 kV/cm electric field，which suggested the lipase activity was greatly improved after treatment by electric field. The improvement of the activity was related to the content increase of β-turn and random coil.

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