“沪农灵芝1号”品种子实体多糖分析

Analysis of Large Molecular Weight Polysaccharide in the Fruiting Bodies of Ganoderma lucidum Cultivar ‘Hunonglingzhi 1’

DOI：10.3969/j.issn.1673-1689.2015.10.012

中文关键词: 灵芝多糖 多角度激光光散射 分子特征

英文关键词: polysaccharide of Ganoderma lucidum,MALLS,molecular character

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中文摘要:

以水提醇沉法获得“沪农灵芝1号”子实体中的灵芝多糖，并对其结构特征和含量进行分析。高效凝胶尺寸排阻色谱-多角度激光光散射仪-示差折光检测仪联用分析结果表明，该多糖重均相对分子质量为2.28×106，均方根半径为150.1 nm，多分散系数（Mw/Mn）为1.14，在水溶液中呈棒状结构。单糖组成及甲基化分析结合酶解试验结果表明，该多糖为β-1，3/1，6-葡聚糖。经液相色谱法定量检测，段木栽培所得灵芝子实体中，该多糖质量分数为0.52 mg/g，显著高于袋料栽培所得子实体中相应组分的含量。

英文摘要:

The high molecular polysaccharide fraction was purified by precipitating a hot-water extract with 20%（V/V） ethanol from fruiting bodies of Ganoderma lucidum cultivar 'Hunonglingzhi 1'. The high performance size exclusion chromatography - multiple angle laser light scattering - refractive index detector（HPSEC-MALLS-RI） was adopted to determine the molecular characteristics of this fraction. Results indicated that the polysaccharide possessed high weight average molecular weight（Mw） of 2.28×106，small polydispersity of 1.14 and large gyration radius（Rg） of 150.1 nm，and existed with a rigid chain conformation in the liquid solution. Structural analysis showed that this polysaccharide was a β-（1，3）/（1，6）-linked D-glucan. Its content determined by HPLC in wood cultivated fruiting bodies was about 0.52 mg/g，which was significantly higher than that in bag cultivated pattern.

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