超声波辅助提取金钗石斛多糖工艺优化研究

Optimization of Ultrasonic-Assisted Extraction of Polysaccharides from Dendrobium nobile Lindl.

DOI：10.3969/j.issn.1673-1689.2015.10.016

中文关键词: 金钗石斛多糖 提取 工艺优化 正交试验

英文关键词: Dendrobium nobile Lindl. polysaccharides,extraction,process optimization,orthogonal test

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中文摘要:

优化超声波辅助提取金钗石斛多糖工艺。以多糖提取得率为考察指标，用苯酚-硫酸法测定多糖的含量，采用单因素试验正交试验法考察了料液比、超声波提取功率、提取温度和提取时间对金钗石斛多糖提取得率的影响。超声波辅助提取金钗石斛多糖的最佳提取工艺条件为：料液质量体积比1 g∶15 mL，超声波提取功率为450 W，超声波提取温度为60 ℃，超声波提取时间为50 min，在此条件下多糖得率为3.11%。

英文摘要:

The study aimed to optimize the ultrasonic-assisted extraction of polysaccharides from Dendrobium nobile Lindl. Using extraction rate as evaluation index，the polysaccharide content was assayed by the phenol-sulfuric method. Based on the orthogonal test，the optimal ultrasonic-assisted extraction conditions were：material-liquid ratio 1∶15（g/mL），extraction power 450 W，temperature 60 ℃ and time 50 min. With the optimal conditions，the extraction rate of polysaccharides was up to 3.11%.

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