食品中马源性成分的实时荧光PCR检测

Detection for Horse-Derived Components in Foods by Real-Time Polymerase Chain Reaction

DOI：10.3969/j.issn.1673-1689.2015.09.010

中文关键词: 马线粒体DNA细胞色素b基因 实时荧光PCR方法 定性检测

英文关键词: horse mitochondrial DNA cytochrome b gene, real-time PCR, qualitative detection

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中文摘要:

针对马线粒体DNA细胞色素b基因设计特异性引物和探针，建立食品中马源性成分实时荧光PCR检测方法，并经特异性和灵敏度试验验证其可行性。结果表明:该体系可扩增马DNA片段，长度为127 bp，其他常见畜、禽肉成分均无法正常扩增。该体系的检测灵敏度为1.25 pg马DNA和质量分数0.001%马肉粉。经市售食品的检测验证，表明所建立的马引物探针体系具有特异性好、灵敏度高、快速、高效等优点，可用于对食品中马源性成分的掺假鉴别检测。

英文摘要:

Species-specific real-time PCR assay was developed for the detection of horse-derived materials in foods. Primers and probes were designed with horse conservative regions of the mitochondrial cytochrome b gene (amplicon=127 bp). The specificity was evaluated and no amplifications on DNA from other meats came under observation. The method was specific for horse-derived materials，and the limit of detection was 1.25 pg of horse DNA and 0.001% (w/w) for horse powder. All these results confirmed that the real-time PCR assay is a rapid，sensitive and specific routine food analysis for the detection of horse in foods.

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