Box-Behnken响应面设计法优化微波辅助提取猪苓多糖工艺

Microwave-Assisted Extraction of Polysaccharides from Polyporus umbellatus Optimized by Box-Behnken Design-Response Surface Methodology

DOI：10.3969/j.issn.1673-1689.2015.09.014

中文关键词: 猪苓 微波辅助提取 多糖 Box-Behnken设计 响应面分析

英文关键词: Polyporus umbellatus, microwave-assisted extraction, polysaccharides, Box-Behnken design, response surface analysis

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中文摘要:

为了优化猪苓多糖的微波提取工艺，采用Box-Behnken响应面设计法，研究液料比、pH、微波功率、提取时间、提取次数及其交互作用对多糖提取率的影响。应用Design Expert和响应面分析相结合的方法，模拟得到回归方程的预测模型和可信度，分析得到最佳提取工艺条件为:液料比30∶1(mL/g)，pH 6.6，微波功率614 W，提取时间2.5 min，提取次数2 次。在此条件下，多糖提取率达到6.75%。利用Box-Behnken响应面设计法优化得到的猪苓多糖提取条件参数，为猪苓多糖工业化生产提供技术支持。

英文摘要:

To optimize the microwave-assisted extraction technique of polysaccharide from Polyporus umbellatus，the effects of the liquid to solid ratio，pH，microwave power，extraction time，extraction number and their interaction on extaction were studied by Box-Behnken design. The predictive model and reliability were developed by Design Expert software and response surface analysis (RSM). The optimal extraction conditions were achieved and listed as follows:liquid-to-solid ratio 30∶1 mL/g，pH 6.6，microwave power 614 W，extraction time 2.5 mins，extraction number 3 times. Under these conditions，the extraction yield of polysaccharide was up to 6.75%. The extraction conditions achieved by RSM provided technical support for the industrial production of polysaccharide from Polyporus umbellatus.

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