菊糖抗氧化活性及其机理
Studies on the Antioxidant Activity of Inulin and Its Mechanism
DOI:10.3969/j.issn.1673-1689.2015.09.016
中文关键词: 菊糖 抗氧化活性 Caco-2细胞模型 机制
英文关键词: Inulin, antioxidant activity, the Caco-2 cell monolayer model, mechanism
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作者
单位
刘德萍
江南大学 直属附属医院(无锡市第四人民医院),江苏 无锡 214062
吴平
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中文摘要:
通过测定菊糖的还原能力、Fe2+螯合能力,以及DPPH自由基清除能力等方法,评价菊糖的抗氧化能力,并采用Caco-2细胞模型探讨其抗氧化机理。3种抗氧化活性实验结果表明,菊糖具有良好的抗氧化活性。与阳性对照组比较,菊糖对H2O2诱导的Caco-2细胞氧化损伤具有显著的保护作用。当菊糖质量浓度为0.8 mg/mL时,Caco-2细胞中过氧化氢酶、谷胱甘肽还原酶,以及谷胱甘肽过氧化物酶的活力,分别提高了35.8%,47.6%和61.2%。菊糖主要通过提高细胞内抗氧化酶的活力发挥其抗氧化作用。
英文摘要:
The antioxidant activity of inulin was evaluated by the studies of the reduction ability,Fe2+-chelating activity and DPPH free radical scavenging activity. The mechanism of its antioxidant activity was investigated using the Caco-2 cell monolayer model. Three different antioxidant activity assays showed that inulin exhibited good antioxidant activity. A significant protective effect of inulin on H2O2 induced oxidative stress in Caco-2 cells was observed in comparison with the positive controls. With the treatment of 0.8 mg/mL insulin,the activities of superoxide dismutase,catalase and glutathione peroxidase were increased by 35.8%,47.6%,and 61.2%,respectively. The antioxidant activity of inulin takes effect mainly by activing the intracellular antioxidant enzymes.
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