蛹虫草Cordyceps militaris JN168菌株的诱变育种及液态发酵产虫草素

Mutation Breeding and Cordycepin Production Study of Cordyceps militaris JN168

DOI：10.3969/j.issn.1673-1689.2015.08.004

中文关键词: 蛹虫草 虫草素 诱变 液态发酵 优化

英文关键词: Cordyceps militaris, cordycepin, mutation, liquid fermentation, optimization

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中文摘要:

利用离子束、亚硝基胍及离子束-亚硝基胍诱变法处理蛹虫草Cordyceps militaris JN168，初步获得了几株较高产虫草素的菌株。通过进一步筛选得到复合诱变菌2，并对该菌的液态发酵培养基组分进行了优化。实验结果表明：蛹虫草液态发酵产虫草素的最佳培养基组分为（g/L），葡萄糖40，酵母浸粉25，MgSO4·7H2O 0.6，K2HPO4·3H2O 0.6，KH2PO4 0.6。优化后虫草素产量提高了5倍，最高达1 045.65 mg/L。

英文摘要:

Cordycepin is the major effective component of Cordyceps militaris，which is getting more and more attention for its hygienic and medical effects. In this study，we obtained several strains with high cordycepin yield from Cordyceps militaris JN168 by using ion beam，nitrosoguanidine and ion beam-nitrosoguanidine mixed mutation. After further screened，the high yield compound mutation strain 2 was obtained. Furthermore，the liquid medium composition of this strain was optimized for the fermentation of cordycepin. The results showed that the components of optimized medium were as follows：glucose 40 g/L，yeast extract 25 g/L，MgSO4·7H2O 0.6 g/L，K2HPO4·3H2O 0.6 g/L，KH2PO4 0.6 g/L. With this optimized medium，the yield of cordycepin increased 5 times，reaching 1 045.65 mg/L.

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