聚乙烯醇降解菌的筛选与降解条件优化

Screening of PVA-Degrading Strains and Optimization of Degradation Conditions

DOI：10.3969/j.issn.1673-1689.2015.08.008

中文关键词: 聚乙烯醇 混合菌系 生物降解 响应面法

英文关键词: polyvinyl alcohol, mixed bacteria, biodegradation, response surface methodology

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中文摘要:

为了实现纺织工业废水中PVA1799的生物降解，从纺织印染厂区筛选到一个能快速降解培养基中PVA1799的混合菌系，命名为SH-TD。从中分离出3株纯培养菌株，分别命名鉴定为SH1 （Aeromonas sp.），TD5 （Pseudomonas sp.），TD33 （Acinetobacter sp.）。通过对含PVA1799出发培养基进行单因素及Placckett-Burman实验筛选出三个对PVA1799降解有显著影响的因素分别为Yeast-Extract、（NH4）2HPO4、CaCl2·2H2O。然后进行最陡爬坡实验逼近最佳响应面区域，最后通过Box-Behnken设计，利用Design-Expert软件进行回归分析，得到各因素的最佳质量浓度组成为（g/L）：Yeast-Extract 0.04、（NH4）2HPO4 48.42、BaCl2 0.06。在最优条件下，24 h PVA降解率从原先的30%提高到80%。

英文摘要:

In order to achieve the biodegradation of PVA1799 in the textile industrial waste water，an bacteria mixture with efficient degradation was screened and named as SH-TD. Three strains with PVA-degrading ability were isolated from the mixed culture SH-TD and nasmed as SH1（Aeromonas sp.），TD5（Pseudomonas sp.），and TD33（Acinetobacter sp.），respectively. The results from the single factor and Plackett-Burman experiments showed that Yeast-Extract，（NH4）2HPO4 and BaCl2 constituents have the significant influence on PVA degrading rate. Through ascent and Box-Behnken experiments，a response surface methodology（RSM） model was constructed. Data were analyzed by use of Design-Expert software. The optimal concentrations（g/L）of the variables were determined as （g/L）：Yeast-Extract 0.04，（NH4）2HPO4 48.42，BaCl2 0.06. Under the optimal conditions，the PVA degradation rate after 24 h increased from 30% to 80% .

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