低温驯化速度对鲫鱼离水储藏酶活性的影响

Effect of Low Temperature Domestication Speed on the Enzyme Acitivity in the Waterless Storage of Crucian

DOI：10.3969/j.issn.1673-1689.2015.08.010

中文关键词: 丙二醛 线粒体 低温驯化 氨基酸

英文关键词: malondialdehyde, mitochondrion amino acids, low temperature acclimation, amino acids

基金项目:

摘要点击次数: 139

全文下载次数: 775

中文摘要:

为研究低温驯化及储藏温度对鲫鱼活体离水后储藏的影响，成年鲫鱼(Carassius carassius) 放置于水箱中，采用每小时 5 ℃和1 ℃速率,由25 ℃匀速降温降至0 ℃，分别在4 ℃和0 ℃冷库中离水储藏24 h，测定鲫鱼新陈代谢及抗氧化相关酶的活力，并探讨低温驯化及离水储藏对鱼体生理状态的影响。结果表明，1 ℃/h降温有利于乳酸脱氢酶（LDH）活力的保存。经过1 ℃/h降温驯化后，0 ℃储藏异柠檬酸脱氢酶活力功能得到了一定保存，脑琥珀酸脱氢酶（SDH）未受影响。丙氨酸氨基转移酶（ALT）活力体现为受储藏温度影响，0 ℃下其活力得到保存。0 ℃储藏导致丙二醛（MDA）的积聚，同时低温储藏中血液过氧化氢酶（CAT）集聚而肝脏未表现出明显变化。

英文摘要:

This work studied the impact of hyperthermia acclimation and storage temperature on waterless storage of live Crucian Carps. Crucian Carps were hyperthermia acclimated at the speed of 5 ℃/h and 1 ℃/h，and then cultured for 24 hours at 4 ℃ and 0 ℃ in waterless ice storage rooms to observe variations activity of metabolic enzyme and antioxidant enzyme. The results showed that the temperature adaption set as 1 ℃/h increased the activity of Lactate Dehydrogenase（LDH）. The activity of isocitrate dehydrogenase（IDH） was maintained comparatively well under such condition，while brain succinate dehydrogenas （SDH） didn't change. Alaninetraensaminase （ALT） activity was maintained at 0 ℃storage，and Malondialdehyde（MDA） acitivity was accumulated at 0 ℃. No obvious change was discovered in liver，which was caused by accumulated Catalase（CAT） low temperature storage.

查看全文 查看/发表评论 下载PDF阅读器