葡萄糖-组胺美拉德产物的生成及对HL-7702细胞生长抑制和细胞周期的影响

Maillard Reaction Between Glucose and Histamine and Its Inhibition on Cell Proliferation and Effect on Cell Cycle of Human Hepatic Cell Line (HL7702)

DOI：10.3969/j.issn.1673-1689.2015.07.006

中文关键词: 葡萄糖-组胺美拉德产物 组胺 人正常肝细胞(HL-7702) 细胞生长抑制 细胞周期

英文关键词: Maillard reaction products(MRPs), histamine, human hepatic cell line(HL7702), inhibition on cell proliferation, cell cycle

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中文摘要:

探讨了葡萄糖-组胺美拉德产物 ( MRPs ) 和组胺( Histamine )对人正常肝细胞(HL-7702)生长抑制和细胞周期可能的作用和影响。用不同剂量的葡萄糖-组胺美拉德产物和组胺处理HL-7702细胞，用MTT方法分别检测美拉德产物和组胺对HL-7702细胞的生长抑制效果；用Annexin检测法，在流式细胞仪下观察两种药物对HL-7702细胞周期分布的影响。MRPs和组胺作用于HL-7702细胞24、48、72 h后，对细胞均有生长抑制作用，其抑制作用呈现剂量和时间依赖性。但组胺对HL-7702细胞的生长抑制作用明显强于MRPs；MRPs产物和组胺两种药物作用于细胞72 h后对细胞周期都有一定的影响，其中组胺的作用明显强于MRPs。葡萄糖和组胺生成的产物能显著消减组胺对HL-7702细胞的杀伤作用。

英文摘要:

The effects of Maillard reaction products(MRPs) and histamine on the inhibition of cell proliferation and cell cycle of human HL7702 were investigated. Human HL 7702 cells were respectively treated by MRPs and histamine with different concentrations for 24，48 and 72 h，i.e.，0.2 mg/mL、0.4 mg/mL、0.6 mg/mL、0.8 mg/mL、1 mg/mL for MRPs，and 0.047 mg/mL、0.094 mg/mL、0.141 mg/mL、0.188 mg/mL、0.235 mg/mL for histamine. The inhibition of cell proliferation induced by MRPs and histamine was detected by MTT assay. The effects of MRPs and histamine on the cell cycle distribution in human HL7702 were detected with flow cytometry using Annexin assay. The inhibition of cell proliferation induced by MRPs and histamine was dependent on the treatment concentration and duration，in which the inhibition was observed after 24 h、48 h、72 h treatment in human HL7702. Histamine was a stronger inhibitor compared with MRPs. The cell cycle arrest was affected by both MRPs and histamine，while histamine was more effective. In conclusion，MRPs could significantly reduce the histamine-induced cytotoxicity of human HL-7702 cells.

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