枯草芽孢杆菌产过氧化氢酶的优化与工业化

Optimization and Industrial Production of Catalase in Bacillus subtilis

DOI：10.3969/j.issn.1673-1689.2015.05.006

中文关键词: 枯草芽孢杆菌 过氧化氢酶 优化 放大实验

英文关键词: Bacillus subtilis,catalase,fermentation,optimization,larger-scale production

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中文摘要:

为了实现过氧化氢酶的工业化生产,作者对实验室前期构造成功的B. subtilis WSHDZ-01 (pSTOP1622-katA)进行了3 L发酵罐的发酵验证,并且通过数据分析优化了碳氮源浓度,使得过氧化氢酶酶活达到35 398 U/mL,比优化前提高53.41%。在此基础上,分别进行了24 L发酵罐和500 L发酵罐的放大实验,最高酶活分别达到28 940、23 190 U/mL。结果表明,该过氧化氢酶在产酶水平上已经达到工业化生产需求,为更大规模的工业化生产奠定坚实的基础。

英文摘要:

Microorganism catalase,which is an important industrial enzyme,plays an important role in textile industry. To realize the industrial production of catalase,the fermentation on B. subtilis WSHDZ-01(pSTOP1622-katA),which was constructed before,was conducted in 3 L fermentor. Furthermore,the enzyme activity reached 35 398 U/mL after optimization of carbon and nitrogen concentration,which was increased by 53.41% compared with that before optimization. Based on this result,the fermentations in 24 L and 500 L fermentor were carried out,respectively and the maximum enzyme activity was 28 940 U/mL and 23 190 U/mL respectively. The result showed the yield of catalase has met the requirement of industrial production,which lay a solid foundation for larger-scale industrial production.

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