采用超滤法、离子交换层析和凝胶过滤层析分离阿魏菇抗氧化多肽,研究其抗氧化性能。以胃蛋白酶-胰蛋白酶联合酶解阿魏菇多肽水解物为原料,采用超滤法分离阿魏菇水解液,使用10 000的超滤膜,将阿魏菇水解液分离成10 000以下的多肽组分和大于10 000的多肽组分,小于10 000的多肽组分的·OH自由基清除能力和DPPH·自由基清除能力较高。小于10 000的多肽组分经DEAE-32纤维素分离,得到3个洗脱峰,其中P3组分的羟基清除能力较高,达到50.22%。P3组分采用葡聚糖凝胶SephadexG-25分离,在220 nm处得到5个洗脱峰,组分P3-2的羟自由基清除最高,达到77.23%,具有较高的抗氧化活性,其相对分子质量大约为1 862。
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Antioxidant peptides of Pleurotus ferulae lenzi were separated using ultra-filtration,ion-exchange and gel filtration chromatography,and its antioxidant properties were also studied in this study. Two kinds of peptides(＞10 000 polypeptide components and ＜10 000 polypeptide components )were isolated from the hydrolyzed solution of Pleurotus ferulae lenzi protein. The polypeptide components under 10 000 have higher ·OH radical scavenging and DPPH· radical scavenging capacity than those above 10 000. After detaching of polypeptide components under 10 000 by DEAE-32,three elution peaks were obtained and P3 component showed higher hydroxyl scavenging capacity,reaching 50.22%. Five elution peaks at 220 nm were further isolated from P3 component by Sephadex G-25,and P3-2 component showed best capacity of ·OH radical scavenging,reaching 77.23%. According to standard equation,the molecular weight of P3-2 was about 1 862. The results provide a theoretical basis for further studies of active ingredient from Pleurotus ferulae lenzi in Xinjiang province.
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