分阶段控制温度提高胞外多糖产量

Two-Stage Temperature Controlling Strategy For Improving the Yield of Expolysaccharide

DOI：10.3969/j.issn.1673-1689.2015.05.016

中文关键词: 分阶段控制温度工艺 胞外多糖 响应面法

英文关键词: two-stage temperature control strategy,expolysaccharide,RSM

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中文摘要:

利用冬虫夏草头孢菌发酵生产胞外多糖,研究不同温度下对菌体生长和胞外多糖产量的影响。结果表明:26 ℃是菌体生长的最适温度,28 ℃是产物积累的最适温度。在此基础上,提出分阶段控制温度工艺。通过响应面法分析,得到优化方案为第一阶段温度26 ℃,变温时间17 h,第二阶段温度28.5 ℃,胞外多糖产量10.92 g/L,比28 ℃恒温培养提高了25.5%。

英文摘要:

The fermentation production of expolysaccharide from Cordyceps militaris was studied at different temperature. The results showed that the optimal growth temperature was at 26 ℃ and the highest yield of expolysaccharide accumlation temperature was at 28 ℃. Thus,the two-stage temperature control strategy was developed based on RSM. When pre-fermentation temperature was kept at 26 ℃ for 17 hours and changed to 28.5 ℃ ,the yield increased to 10.92 g/L which was 25.5% higher than that of fermentation at single temperature at 28 ℃.

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